F1的γ亚基和F0的PVP蛋白（F0I）是线粒体F0F1 H(+) -ATP合酶门控的组成部分。
The gamma subunit of F1 and the PVP protein of F0 (F0I) are components of the gate of the mitochondrial F0F1 H(+)-ATP synthase.
作者信息
Papa S, Guerrieri F, Zanotti F, Fiermonte M, Capozza G, Jirillo E
机构信息
Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.
出版信息
FEBS Lett. 1990 Oct 15;272(1-2):117-20. doi: 10.1016/0014-5793(90)80462-r.
The gamma subunit of the F1 moiety of the bovine mitochondrial H(+)-ATP synthase is shown to function as a component of the gate. Addition of purified gamma subunit to F0-liposomes inhibits transmembrane proton conduction. This inhibition can be removed by the bifunctional thiol reagent diamide. Immunoblot analysis shows that the diamide effect is likely due to disulphide bridging of the gamma subunit with the PVP protein of the F0 sector.
牛线粒体H(+)-ATP合酶F1部分的γ亚基被证明作为门控组件发挥作用。将纯化的γ亚基添加到F0脂质体中会抑制跨膜质子传导。这种抑制作用可被双功能硫醇试剂二酰胺消除。免疫印迹分析表明，二酰胺的作用可能是由于γ亚基与F0区段的PVP蛋白形成二硫键桥接所致。
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