使用锰增强磁共振成像观察维拉帕米对大鼠脑嗅觉连接的影响的统计参数映射。

Statistical parametric mapping for effects of verapamil on olfactory connections of rat brain in vivo using manganese-enhanced MR imaging.

机构信息

Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.

出版信息

Magn Reson Med Sci. 2011;10(2):107-19. doi: 10.2463/mrms.10.107.

DOI:10.2463/mrms.10.107

PMID:21720113

Abstract

PURPOSE

We investigated the effect of verapamil on the transport of manganese in the olfactory connections of rat brains in vivo using statistical parametric mapping and manganese-enhanced magnetic resonance (MR) imaging.

METHODS

We divided 12 7-week-old male Sprague-Dawley rats into 2 groups of six and injected 10 μL of saline into the right nasal cavities of the first group and 10 μL of verapamil (2.5 mg/mL) into the other group. Twenty minutes after the initial injection, we injected 10 μL of MnCl(2) (1 mol/L) into the right nasal cavities of both groups. We obtained serial T(1)-weighted MR images before administering the verapamil or saline and at 0.5, one, 24, 48, and 72 hours and 7 days after administering the MnCl(2), spatially normalized the MR images on the rat brain atlas, and analyzed the data using voxel-based statistical comparison.

RESULTS

Statistical parametric maps demonstrated the transport of manganese. Manganese ions created significant enhancement (t-score = 36.6) 24 hours after MnCl(2) administration in the group administered saline but not at the same time point in the group receiving verapamil. The extent of significantly enhanced regions peaked at 72 hours in both groups and both sides of the brain. The peak of extent in the right side brain in the group injected with saline was 70.2 mm(3) and in the group with verapamil, 92.4 mm(3). The extents in the left side were 64.0 mm(3) for the group with saline and 53.2 mm(3) for the group with verapamil.

CONCLUSION

We applied statistical parametric mapping using manganese-enhanced MR imaging to demonstrate in vivo the transport of manganese in the olfactory connections of rat brains with and without verapamil and found that verapamil did affect this transport.

摘要

目的

我们采用统计参数图和锰增强磁共振成像（MR），研究维拉帕米对活体大鼠嗅神经通路内锰转运的影响。

方法

我们将 12 只 7 周龄雄性 Sprague-Dawley 大鼠随机分为 2 组，每组 6 只。第 1 组右侧鼻腔注射 10 μL 生理盐水，第 2 组右侧鼻腔注射 10 μL 维拉帕米（2.5 mg/mL）。初次注射 20 min 后，两组右侧鼻腔均注射 10 μL 1 mol/L 的 MnCl2。在注射维拉帕米或生理盐水前、注射 MnCl2 后 0.5、1、24、48、72 h 和 7 d 时，我们获得一系列 T1 加权 MR 图像，将 MR 图像在大鼠脑图谱上进行空间标准化，并采用基于体素的统计比较进行数据分析。

结果

统计参数图显示了锰的转运。注射 MnCl2 24 h 后，注射生理盐水组的锰离子引起明显增强（t 评分=36.6），但注射维拉帕米组未观察到同一时间点的增强。两组大脑双侧均在 72 h 时达到增强区域的峰值，峰值在两组中持续到 7 天。注射生理盐水组右侧大脑的峰值为 70.2 mm3，注射维拉帕米组为 92.4 mm3。注射生理盐水组左侧大脑的峰值为 64.0 mm3，注射维拉帕米组为 53.2 mm3。