Awazu Kaoru, Nomura Chie, Yamaguchi Mizuka, Obana Hirotaka
Osaka Prefectural Institute of Public Health.
Shokuhin Eiseigaku Zasshi. 2011;52(3):199-204. doi: 10.3358/shokueishi.52.199.
A simple and practical method was developed for the determination of histamine in fish and fish products by solid-phase extraction and fluorescence derivatization. Histamine was extracted with trichloroacetic acid. The extract was neutralized and diluted with phosphate buffer (pH 6.8), and cleaned up with a tandem-connected octadecyl silica (ODS) and strong cation exchange silica (SCX) cartridge. After removal of the solvent, histamine was derivatized with fluorescamine and analyzed by ion-paired reversed-phase high-performance liquid chromatography with fluorescence detection. Recovery tests of histamine from six kinds of fish and fish products showed acceptable recovery (83-92%) with low relative standard deviation (less than 5%). This method could be useful for determination of histamine in fish.
建立了一种简单实用的固相萃取-荧光衍生化法测定鱼类及鱼制品中的组胺。组胺用三氯乙酸提取。提取物用磷酸盐缓冲液（pH 6.8）中和并稀释，然后用串联连接的十八烷基硅胶（ODS）和强阳离子交换硅胶（SCX）柱净化。除去溶剂后，组胺用荧光胺衍生化，并用离子对反相高效液相色谱-荧光检测法进行分析。对六种鱼类及鱼制品中组胺的回收率测试表明，回收率良好（83-92%），相对标准偏差较低（小于5%）。该方法可用于鱼类中组胺的测定。
