Müller G M, Scherf H P, Meffert H, Kohl A, Volk H D
Institut für Medizinische Immunologie, Humboldt-Universität zu Berlin.
Dermatol Monatsschr. 1990;176(5-6):293-304.
Before and after skin treatment by ultraviolet radiation, infrared radiation or visible light the ability of polymorphonuclear leukocytes (PMN) to form oxygen intermediates was investigated in 31 healthy volunteers. Superoxide production was evaluated photometrically as the reduction of Iodonitrotetrazoliumchloride (INT). After whole body treatment by 4 UV exposures all persons exhibited significantly enhanced rates of INT-reduction for 26-62 days. The in vivo half-time of PMN is 5-7 hours. Therefore a direct effect of irradiation over that long period is rather unlikely, suggesting that mediators should play a crucial role in signal transduction from the skin to the peripheral blood cells. Most likely candidates for connective links are interferon-gamma (IFN-gamma), granulocyte-monocyte colony stimulating factor (GM-CSF) and tumor necrosis factor (TNF).
在31名健康志愿者身上，研究了紫外线、红外线或可见光皮肤治疗前后多形核白细胞（PMN）形成氧中间体的能力。通过氯化碘硝基四氮唑（INT）的还原光度法评估超氧化物的产生。经过4次紫外线全身照射后，所有人在26至62天内INT还原率均显著提高。PMN在体内的半衰期为5至7小时。因此，在如此长的时间内辐射产生直接影响的可能性相当小，这表明介质在从皮肤到外周血细胞的信号转导中应起关键作用。最有可能的连接介质候选物是γ-干扰素（IFN-γ）、粒细胞-单核细胞集落刺激因子（GM-CSF）和肿瘤坏死因子（TNF）。
