Zhu Ling, Huq Enamul
Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, The University of Texas, Austin, TX, USA.
Methods Mol Biol. 2011;754:167-84. doi: 10.1007/978-1-61779-154-3_9.
Transcription factors are modular in nature in all organisms. In general, they have a DNA binding domain, one or more transcription activation and/or repressor domain, and often a dimerization domain. In many cases, transcription factors also have other protein-protein interaction domain(s). Mapping these functional domains in transcription factors is critical in understanding their molecular function. In this chapter, protocols for mapping the DNA binding domain and the transcription activation domain of a bHLH class of transcription factor are described. In principle, these protocols can be applied to other classes of transcription factors for mapping their functional domains.
在所有生物体中，转录因子本质上都是模块化的。一般来说，它们具有一个DNA结合结构域、一个或多个转录激活和/或抑制结构域，并且通常还有一个二聚化结构域。在许多情况下，转录因子还具有其他蛋白质-蛋白质相互作用结构域。确定转录因子中的这些功能结构域对于理解它们的分子功能至关重要。在本章中，描述了用于确定bHLH类转录因子的DNA结合结构域和转录激活结构域的实验方案。原则上，这些实验方案可应用于其他类别的转录因子以确定它们的功能结构域。
