Batra S K, Brown S M
Institute of Virology, Glasgow, Scotland.
Indian J Med Res. 1990 Jul;91:252-7.
Using the rabbit eye model of latency, herpes simplex virus type 1 strain McKrae invariably reactivated after epinephrine iontophoresis, whereas type 2(HSV-2) virus strain HG 52 failed to reactivate. Both strains established a latent infection with the same frequency. To identify the viral genes involved in this reactivation difference, intertypic recombinants were selected following cotransfection of intact McKrae DNA and Xba I or Hpa I cleaved HG52 DNA. Eleven separately obtained recombinants containing HG52 inserts between 0.35-0.56 and/or 0.82-1.0 map units (mu) were isolated but as four that were tested reactivated with the same frequency as the parental Mckrae virus, it was established that the genes encoded between these map coordinates do not determine the reactivation difference.
利用兔眼潜伏模型，单纯疱疹病毒1型McKrae株在肾上腺素离子导入后总是重新激活，而2型（HSV-2）病毒株HG 52未能重新激活。两种病毒株建立潜伏感染的频率相同。为了鉴定与这种重新激活差异相关的病毒基因，在完整的McKrae DNA与经Xba I或Hpa I切割的HG52 DNA共转染后选择了种间重组体。分离出11个单独获得的重组体，其在0.35-0.56和/或0.82-1.0图谱单位（mu）之间含有HG52插入片段，但由于测试的4个重组体与亲本McKrae病毒以相同频率重新激活，因此确定在这些图谱坐标之间编码的基因并不能决定重新激活差异。
