Kujala N G, Karanfil C, Barrea R A
Biophysics Collaborative Access Team (BioCAT), Department of Biological Chemical, and Physical Sciences, Illinois Institute of Technology, Chicago, Illinois 60616, USA.
Rev Sci Instrum. 2011 Jun;82(6):063106. doi: 10.1063/1.3595675.
We have developed a compact short focal distance Bent Crystal Laue Analyzer (BCLA) for Cu speciation studies of biological systems with specific applications to cancer biology. The system provides high energy resolution and high background rejection. The system is composed of an aluminum block serving as a log spiral bender for a 15 micron thick Silicon 111 crystal and a set of soller slits. The energy resolution of the BCLA-about 14 eV at the Cu Kα line- allows resolution of the Cu Kα(1) and CuKα(2) lines. The system is easily aligned by using a set of motorized XYZ linear stages. Two operation modes are available: incident energy scans (IES) and emission energy scans (EES). IES allows scanning of the incident energy while the BCLA system is maintained at a preselected fixed position--typically CuKα(1) line. EES is used when the incident energy is fixed and the analyzer is scanned to provide the peak profile of the emission lines of Cu.
我们开发了一种紧凑的短焦距弯曲晶体劳厄分析仪（BCLA），用于生物系统中铜形态的研究，并特别应用于癌症生物学。该系统具有高能量分辨率和高背景抑制能力。它由一个铝块组成，该铝块用作15微米厚的硅111晶体的对数螺旋弯曲器以及一组索勒狭缝。BCLA在铜Kα线处的能量分辨率约为14电子伏特，这使得能够分辨出铜Kα(1)线和铜Kα(2)线。通过使用一组电动XYZ线性平台，该系统易于对准。有两种操作模式：入射能量扫描（IES）和发射能量扫描（EES）。IES允许在BCLA系统保持在预选的固定位置（通常是铜Kα(1)线）时扫描入射能量。当入射能量固定且扫描分析仪以提供铜发射线的峰值轮廓时，使用EES。
