Wu T C, Mann R B, Charache P, Hayward S D, Staal S, Lambe B C, Ambinder R F
Department of Pathology, Johns Hopkins Medical School, Baltimore, MD 21205.
Int J Cancer. 1990 Nov 15;46(5):801-4. doi: 10.1002/ijc.2910460509.
EBV DNA has been detected by Southern blot hybridization in 20-25% of Hodgkin's disease tumor specimens and localized to the Reed-Sternberg cells by in situ hybridization. In the present investigation we used a 3H-labelled EBER I anti-sense RNA for in situ hybridization of archival formalin-fixed paraffin-embedded Hodgkin's disease specimens previously shown by Southern Blot hybridization to be EBV-positive. In 6 of 8 specimens neoplastic cells showed an intense signal in virtually all of the tumor cells. The background lymphocytes, eosinophils, plasma cells and histiocytes did not demonstrate significant hybridization. In each case hybridization tended to spare the nucleoli. Hybridization was detected in specimens with histologies of mixed cellularity and nodular sclerosis. The intensity of signal relative to background is better than that in previous studies utilizing 35S-labelled large internal repeat probes for EBV in Reed-Sternberg cells. The exposure time is one week in contrast to the 4-5 weeks reported by others for detection of EBV in Hodgkin's disease. Both increased relative intensity and shorter exposure requirements may be attributed to the very high number of EBER transcripts in the target cells. The demonstration that EBER I is expressed is consistent with a role for EBV in growth regulation of Reed-Sternberg cells and suggests that the virus is not merely a silent passenger in Hodgkin's disease.
通过Southern印迹杂交在20%-25%的霍奇金病肿瘤标本中检测到EBV DNA，并通过原位杂交将其定位于里德-施特恩伯格细胞。在本研究中，我们使用3H标记的EBER I反义RNA对存档的福尔马林固定石蜡包埋的霍奇金病标本进行原位杂交，这些标本先前通过Southern印迹杂交显示为EBV阳性。在8个标本中的6个中，肿瘤细胞在几乎所有肿瘤细胞中都显示出强烈信号。背景淋巴细胞、嗜酸性粒细胞、浆细胞和组织细胞未显示出明显的杂交信号。在每种情况下，杂交往往避开核仁。在混合细胞型和结节硬化型组织学的标本中检测到杂交信号。相对于背景的信号强度优于先前在里德-施特恩伯格细胞中利用35S标记的EBV大内部重复探针进行的研究。曝光时间为一周，而其他人报道在霍奇金病中检测EBV的曝光时间为4-5周。相对强度增加和曝光时间缩短可能都归因于靶细胞中EBER转录本数量非常高。EBER I表达的证明与EBV在里德-施特恩伯格细胞生长调节中的作用一致，并表明该病毒在霍奇金病中不仅仅是一个沉默的过客。
