Dilipkumar Shilpa, Diaspro Alberto, Mondal Partha Pratim
Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore 560012, India.
Rev Sci Instrum. 2011 Jun;82(6):063705. doi: 10.1063/1.3600795.
Three-dimensional (3D) resolution improvement in multi-photon multiple-excitation-spot-optical microscopy is proposed. Specially designed spatial filter is employed for improving the overall 3D resolution of the imaging system. An improvement up to a factor of 14.5 and sub-femto liter volume excitation is achieved. The system shows substantial sidelobe reduction (<4%) due to the non-linear intensity dependence of multiphoton process. Polarization effect on x-oriented and freely rotating dipoles shows dramatic change in the field distribution at the focal-plane. The resulting point-spread function has the ability to produce several strongly localized polarization dependent field patterns which may find applications in optical engineering and bioimaging.
提出了在多光子多激发点光学显微镜中提高三维（3D）分辨率的方法。采用专门设计的空间滤波器来提高成像系统的整体三维分辨率。实现了高达14.5倍的分辨率提升以及亚飞升体积激发。由于多光子过程的非线性强度依赖性，该系统的旁瓣大幅降低（<4%）。偏振对x方向和自由旋转偶极子的影响在焦平面的场分布中显示出显著变化。由此产生的点扩散函数能够产生几种强烈局域化的偏振相关场模式，这可能在光学工程和生物成像中得到应用。
