儿童下呼吸道定植生殖器支原体属。

Colonization of paediatric lower respiratory tract with genital Mycoplasma species.

机构信息

Department of Microbiology, University of Massachusetts, Amherst Department of Pediatric Pulmonology, Baystate Medical Center, Springfield, Massachusetts, USA.

出版信息

Respirology. 2011 Oct;16(7):1081-7. doi: 10.1111/j.1440-1843.2011.02016.x.

DOI:10.1111/j.1440-1843.2011.02016.x

PMID:21722263

Abstract

BACKGROUND AND OBJECTIVE

Recently, much attention has been given to the possible role played by pathogens that colonize neonatal or paediatric airway and their potential involvement in chronic respiratory disease. The goal of the current study was to evaluate the prevalence of Mycoplasma organisms in the BAL fluid of paediatric patients suffering from a variety of chronic respiratory diseases to determine if there was any clear disease association with bacterial presence.

METHODS

We examined 319 paediatric BAL samples for the presence of M.genitalium, M.hominis, U.urealyticum, U.parvum and M.pneumoniae DNA with species-specific PCR.

RESULTS

Mycoplasma DNA was found in 32.6% (104/319) of patient samples; 10% (32/319) for M.pneumoniae, 8.8% for U.parvum, 2.8% for U.urealyticum; 4.7% for M.hominis and 9.1% for M.genitalium. There were no significant clinical and laboratory differences except serum IgE in asthmatics according to Mycoplasma colonization or not. Elevated levels of IgE were found more often in Mycoplasma DNA-negative patients than patients with bacterial DNA, 85/109 versus 24/109 respectively (P<0.0001). There was no difference in the frequency of Mycoplasmas between the asthmatics and the non-asthmatics; 30.6% (69/225) versus 37.2% (35/94) for Mycoplasma 16S DNA, and 8% versus 14.9% for M.pneumoniae, respectively.

CONCLUSIONS

Our data indicate that in addition to M.pneumoniae, urogenital Mycoplasma species may colonize the airway of patients with chronic respiratory diseases. There was, however, no association between chronic asthma diagnosis and Mycoplasma colonization in this study.

摘要

背景与目的

最近，人们对定植于新生儿或儿科气道的病原体及其在慢性呼吸道疾病中的潜在作用给予了极大关注。本研究旨在评估各种慢性呼吸道疾病患儿 BAL 液中支原体生物体的流行率，以确定细菌存在与疾病之间是否存在明确关联。

方法

我们使用种特异性 PCR 检测了 319 份儿科 BAL 样本中 M. genitalium、M. hominis、U. urealyticum、U. parvum 和 M. pneumoniae 的 DNA。

结果

在 319 份患者样本中发现支原体 DNA 占 32.6%（104/319）；其中 M. pneumoniae 占 10%（32/319）、U. parvum 占 8.8%、U. urealyticum 占 2.8%、M. hominis 占 4.7%、M. genitalium 占 9.1%。除哮喘患者根据有无支原体定植存在血清 IgE 差异外，支原体定植与临床和实验室结果无显著相关性。支原体 DNA 阴性患者的 IgE 水平高于 DNA 阳性患者，分别为 85/109 与 24/109（P<0.0001）。支原体在哮喘患者与非哮喘患者中的检出率无差异：16S DNA 分别为 30.6%（69/225）与 37.2%（35/94），M. pneumoniae 分别为 8%与 14.9%。