Fonseca-Camarillo G, Furuzawa-Carballeda J, Martínez-Benítez B, Barreto-Zúñiga R, Yamamoto-Furusho K
Clínica de Enfermedad Inflamatoria Intestinal, Departamento de Gastroenterología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México, D. F.
Rev Gastroenterol Mex. 2011 Apr-Jun;76(2):113-9.
Interleukin-10 (IL-10) is an important immunoregulatory cytokine that acts on antigen presenting cells by the inhibiting both the synthesis of cytokines, co-stimulatory and HLA class II molecules.
To study the gene and protein expression of IL-10 in the mucosa from patients with ulcerative colitis (UC).
We studied 40 patients with UC and 18 controls without endoscopic evidence of intestinal inflammation. From rectal biopsies was determined the gene expression of IL- 10 by real time polymerase chain reaction (PCR). The detection of the protein in tissue was performed by immunohistochemistry.
patients with UC in remission had significantly higher expression of il-10 gene in mucosa compared to the group of patients with active UC (p = 0.01) and the control group (p = 0.05). All patients with active UC had pancolitis, while patients in remission from distal inflammation, 16 had extra-intestinal manifestations and 23 had mild to moderate inflammation with less than one relapse within a year. Patients with UC in remission had significantly higher expression of IL-10 gene in mucosa compared with the group of patients with active UC (p = 0.01) or the control group (p = 0.05).
The expression of IL-10 gene is increased in colonic mucosa from patients with UC in remission, confirming that it is an immunoregulatory cytokine that promotes remission in patients with UC.
白细胞介素-10（IL-10）是一种重要的免疫调节细胞因子，它通过抑制细胞因子的合成、共刺激分子和HLA II类分子，作用于抗原呈递细胞。
研究溃疡性结肠炎（UC）患者黏膜中IL-10的基因和蛋白表达。
我们研究了40例UC患者和18例无肠道炎症内镜证据的对照者。通过实时聚合酶链反应（PCR）从直肠活检组织中测定IL-10的基因表达。组织中蛋白的检测采用免疫组织化学法。
与活动期UC患者组（p = 0.01）和对照组（p = 0.05）相比，缓解期UC患者黏膜中IL-10基因表达显著更高。所有活动期UC患者均为全结肠炎，而缓解期患者中，16例有远端炎症，16例有肠外表现，23例有轻度至中度炎症，一年内复发少于一次。与活动期UC患者组（p = 0.01）或对照组（p = 0.05）相比，缓解期UC患者黏膜中IL-10基因表达显著更高。
缓解期UC患者结肠黏膜中IL-10基因表达增加，证实其是促进UC患者缓解的免疫调节细胞因子。
