Zebrafish Genetics Laboratory, Discipline of Genetics, School of Molecular and Biomedical Sciences, The University of Adelaide.
Dev Genes Evol. 2011 Aug;221(3):179-86. doi: 10.1007/s00427-011-0367-3. Epub 2011 Jul 3.
Klotho is an aging suppressor gene. In mice, loss of Klotho function causes accelerated aging while increased Klotho expression increases longevity. This study aimed to identify and characterize the orthologue of Klotho in zebrafish, a powerful model organism for the investigation of development and human disease. Zebrafish klotho was identified by a bioinformatics approach, and cloning and sequencing of klotho cDNA confirmed the in silico analysis. The zebrafish Klotho protein has a structure similar to human and mouse Klotho, but it lacks an apparent secretory signal sequence. We can find no evidence of an alternative transcript isoform lacking the transmembrane domain coding sequence as seen in mammals. RT-PCR revealed the expression of klotho during embryonic development and in a wider variety of adult tissues than in mouse. Quantitative real-time RT-PCR demonstrated the relative gene expression profile of zebrafish Klotho during embryogenesis and in adult tissues. In situ hybridization showed an apparently diffuse signal of klotho mRNA expression in the adult zebrafish testis.
Klotho 是一种衰老抑制基因。在小鼠中，Klotho 功能缺失会导致加速衰老，而 Klotho 表达增加则会延长寿命。本研究旨在鉴定和描述斑马鱼中 Klotho 的同源物，斑马鱼是一种强大的模型生物，可用于研究发育和人类疾病。通过生物信息学方法鉴定了斑马鱼 klotho，并通过克隆和测序 klotho cDNA 证实了计算机分析。斑马鱼 Klotho 蛋白的结构与人和鼠 Klotho 相似，但缺乏明显的分泌信号序列。我们没有发现证据表明存在缺失跨膜结构域编码序列的替代转录本异构体，如在哺乳动物中所见。RT-PCR 显示 klotho 在胚胎发育过程中和比在小鼠中更多的成年组织中表达。定量实时 RT-PCR 显示了斑马鱼 Klotho 在胚胎发生和成年组织中的相对基因表达谱。原位杂交显示成年斑马鱼睾丸中 klotho mRNA 表达的信号明显弥散。
