Department of Pharmacology, Nanjing University of Chinese Medicine, Nanjing 210029, China.
Chin J Integr Med. 2011 Jul;17(7):537-41. doi: 10.1007/s11655-011-0788-x. Epub 2011 Jul 3.
San'ao Decoction (, SAD), as a representative Chinese medicine (CM) formula, was chosen to evaluate the effect of airway inflammation and hyperresponsiveness on the lipopolysaccharide (LPS) enhanced asthma model.
The asthma model was reproduced in the Balb/C mice sensitized by ovalbumin (OVA), challenged by OVA and LPS. After Balb/C mice's administration of a dose (0.0024 g/kg) of dexamethasone acetate, and three doses (2.2 g/kg, 4.4 g/kg and 8.8 g/kg) of SAD, airway inflammation and responsiveness were observed. The airway inflammation was detected by counting bronchoalveolar lavage fluid (BALF) cells and lung histopathology. Also, differential expressions of interferon-r (IFN-γ), interleukin-4 (IL-4), and IL-5 in the supernatants of BALF were examined. The changes in airway responsiveness indicated by lung resistance (R(L)) and stimulated by acetylcholine (Ach) were determined.
Small-dose SAD hardly inhibit airway inflammation or hyperresponsiveness in the LPS-enhanced asthma, while medium-dose and high-dose SAD significantly inhibited the airway hyperresponsiveness, and to some extent, reduced airway inflammation. Meanwhile, the small-dose, medium-dose, and high-dose SAD promoted Th1-type cytokines (IFN-γ) and reduced Th2-type cytokines (IL-4, IL-5) to different extents, which led to a Th1/Th2 balance.
SAD has a good therapeutic effect on airway hyperresponsiveness in the LPS-enhanced asthma model, but its definite influence on airway inflammation is not remarkable.
三拗汤（SAD）作为一种中药（CM）代表方剂，被选择用于评估气道炎症和高反应性对脂多糖（LPS）增强哮喘模型的影响。
采用卵清蛋白（OVA）致敏Balb/C 小鼠，OVA 和 LPS 激发制作哮喘模型。通过醋酸地塞米松灌胃给药（0.0024 g/kg），以及 SAD 灌胃给药（2.2 g/kg、4.4 g/kg 和 8.8 g/kg）后，观察气道炎症和气道高反应性。通过计数支气管肺泡灌洗液（BALF）细胞和肺组织病理学观察气道炎症。同时，检测 BALF 上清液中干扰素-r（IFN-γ）、白细胞介素-4（IL-4）和白细胞介素-5（IL-5）的差异表达。通过乙酰胆碱（Ach）刺激测定肺阻力（R(L)）变化来指示气道高反应性的变化。
小剂量 SAD 几乎不能抑制 LPS 增强哮喘中的气道炎症或气道高反应性，而中剂量和高剂量 SAD 显著抑制气道高反应性，并在一定程度上减轻气道炎症。同时，小剂量、中剂量和高剂量 SAD 不同程度地促进 Th1 型细胞因子（IFN-γ）并减少 Th2 型细胞因子（IL-4、IL-5），从而导致 Th1/Th2 平衡。
SAD 对 LPS 增强哮喘模型中的气道高反应性有较好的治疗作用，但对气道炎症的确切影响不显著。
