Instrumental Laboratory, Technological National Center of Canning Industry (CTC), Murcia, Spain.
J Environ Sci Health B. 2011;46(6):530-4. doi: 10.1080/03601234.2011.583874.
An analytical procedure has been developed for the determination of natural pyrethrins (pyrethrin I, pyrethrin II, jasmolin I, jasmolin II, cinerin I and cinerin II) in lemon and apricot. The QuEChERS method, which stands for quick, easy, cheap, effective, rugged and safe was used for extraction. Analysis of the extract was performed by liquid-chromatography-electrospray ionisation-tandem mass spectrometry. The ions prominent in the ESI spectra were [M+H]+ for the six compounds. A Zorbax SB-C18 column was used with a programmed gradient mobile phase consisting of (A) water containing 0.1% formic acid and 5 mM ammonium formate and (B) ACN. The method was linear within the investigated concentration range, displaying a calibration curve correlation factor of 0.99. The coefficients of variation obtained (RSD) were below 20% and the recoveries were in the 70-110% range.
已经开发出一种用于测定柠檬和杏仁中天然除虫菊素（除虫菊 I、除虫菊 II、茉莉酮酸 I、茉莉酮酸 II、氰戊菊酸 I 和氰戊菊酸 II）的分析程序。该方法采用 QuEChERS 方法（快速、简便、廉价、有效、耐用和安全）进行提取。通过液相色谱-电喷雾串联质谱法对提取物进行分析。ESI 谱图中突出的离子为[M+H]+，用于六种化合物。使用 Zorbax SB-C18 柱，采用含有 0.1%甲酸和 5 mM 甲酸铵的水（A）和 ACN（B）的程序梯度流动相。该方法在所研究的浓度范围内呈线性，校准曲线相关系数为 0.99。得到的变异系数（RSD）低于 20%，回收率在 70-110%范围内。
