Biophysics Laboratory, Faculty of Medicine of Monastir, 5019 Monastir, Tunisia.
Talanta. 2011 Aug 15;85(2):927-35. doi: 10.1016/j.talanta.2011.04.079. Epub 2011 May 11.
Specific recognition between two biological partners is widely exploited in biosensors nowadays. To explore this avenue, a novel biosensor for antithrombin (AT) detection was constructed. Heparin was used as the affinity ligand. A well-known acrylic monomer (butyl methacrylate) was polymerized and grafted onto the heparin polysaccharide by the use of ceric ammonium nitrate as a redox initiator in aqueous nitric acid medium. Polymers were deposited as a thin layer onto surface of stainless steel electrode (SS316L). The obtained polymers were studied by Fourier transform infrared spectroscopy (FTIR) and analyzed by differential scanning calorimetry (DSC). Moreover, the films were characterized by electrochemical impedance spectroscopy (EIS), contact-angle measurements and AFM. EIS was used to study the biosensor affinity to AT and the relationship between functionalization growth of modified electrode and the response of the sensor. The proposed approach appears to be simple, sensitive and correlated with methods that analyse the detection of antithrombin.
如今，生物传感器广泛利用两种生物配体之间的特异性识别。为了探索这一途径，构建了一种新型的抗凝血酶（AT）检测生物传感器。肝素被用作亲和配体。使用硝酸水溶液中的硝酸铈铵作为氧化还原引发剂，将一种著名的丙烯酸单体（甲基丙烯酸丁酯）聚合并接枝到肝素多糖上。聚合物作为薄层沉积在不锈钢电极（SS316L）的表面上。通过傅里叶变换红外光谱（FTIR）和差示扫描量热法（DSC）对获得的聚合物进行研究。此外，还通过电化学阻抗谱（EIS）、接触角测量和原子力显微镜（AFM）对薄膜进行了表征。EIS 用于研究生物传感器与 AT 的亲和性以及修饰电极的功能化生长与传感器响应之间的关系。该方法简单、灵敏，并与分析抗凝血酶检测的方法相关。
