Biosystems Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark, 4000 Roskilde, Denmark.
Appl Biochem Biotechnol. 2011 Oct;165(3-4):1010-23. doi: 10.1007/s12010-011-9316-x. Epub 2011 Jul 5.
The potential of wheat straw for ethanol production after pretreatment with O(3) generated in a plasma at atmospheric pressure and room temperature followed by fermentation was investigated. We found that cellulose and hemicellulose remained unaltered after ozonisation and a subsequent washing step, while lignin was degraded up to 95% by O(3). The loss of biomass after washing could be explained by the amount of lignin degraded. The washing water of pretreated samples (0-7 h) was analyzed for potential fermentation inhibitors. Approximately 30 lignin degradation products and a number of simple carboxylic acids and phenolic compounds were found, e.g., vanillic acid, acetic acid, and formic acid. Some components had the highest concentration at the beginning of the ozonisation process (0.5, 1 h), e.g., 4-hydroxybenzladehyde, while the concentration of others increased during the entire pretreatment (0-7 h), e.g., oxalic acid and acetovanillon. Interestingly, washing had no effect on the ethanol production with pretreatment times up to 1 h. Washing improved the glucose availability with pretreatment times of more than 2 h. One hour of ozonisation was found to be optimal for the use of washed and unwashed wheat straw for ethanol production (maximum ethanol yield, 52%). O(3) cost estimations were made for the production of ethanol at standard conditions.
研究了在大气压和室温下的等离子体中生成的 O(3)预处理后，小麦秸秆用于生产乙醇的潜力，然后进行发酵。我们发现，臭氧氧化和随后的洗涤步骤后，纤维素和半纤维素保持不变，而木质素的降解率高达 95%。洗涤后生物质的损失可以用降解的木质素量来解释。预处理样品（0-7 h）的洗涤水被分析是否有潜在的发酵抑制剂。大约有 30 种木质素降解产物和一些简单的羧酸和酚类化合物被发现，例如香草酸、乙酸和甲酸。一些成分在臭氧氧化过程的开始时（0.5、1 h）具有最高的浓度，例如 4-羟基苯甲醛，而其他成分在整个预处理过程中（0-7 h）增加，例如，草酸和乙酰香草醛。有趣的是，在预处理时间长达 1 h 的情况下，洗涤对乙醇生产没有影响。洗涤增加了预处理时间超过 2 h 的葡萄糖的可用性。发现臭氧氧化 1 小时对于使用洗涤和未洗涤的小麦秸秆生产乙醇是最佳的（最大乙醇产量为 52%）。在标准条件下生产乙醇的 O(3)成本估算也已做出。
