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对四个蛋鸡品种的线粒体 D 环区进行等位基因特异性 PCR 分型和测序。

Allele-specific PCR typing and sequencing of the mitochondrial D-loop region in four layer breeds.

机构信息

Animal Genome Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Japan.

出版信息

Anim Sci J. 2011 Apr;82(2):223-6. doi: 10.1111/j.1740-0929.2010.00831.x. Epub 2011 Mar 2.

DOI:10.1111/j.1740-0929.2010.00831.x
PMID:21729199
Abstract

This study aimed to investigate the ability of single nucleotide polymorphism (SNP) haplotypes in chicken mtDNA for presumption of the origins of chicken meat. We typed five SNPs of the D-loop region in mtDNA by allele-specific PCR (AS-PCR) in 556 hens, that is 233 White Leghorn (WL), 50 Dekalb-TX35 (D-TX), 140 Barred Plymouth Rock (BPR) and 133 Rhode Island Red (RIR) kept in the National Institute of Livestock and Grassland Science (NILGS, Tsukuba, Japan). Five haplotypes were observed among those chickens by AS-PCR. WL, D-TX, BPR and RIR displayed three, two, one and four SNP haplotypes, respectively. By a combination of the haplotypes by AS-PCR and the breeds, these chickens were classified into 10 groups. After the D-loop was sequenced in two chickens from every group (20 individuals), 15 SNP sites (including one insertion) and eight sequence haplotypes were observed. In conclusion, haplotype variation was observed in and among the layer breeds of the NILGS. This study demonstrates that SNP haplotypes in mtDNA should be appropriate for the presumption of the origins of chicken meat.

摘要

本研究旨在探讨鸡 mtDNA 中单核苷酸多态性 (SNP) 单倍型在推断鸡肉起源方面的能力。我们通过等位基因特异性 PCR (AS-PCR) 在 556 只母鸡中对 mtDNA 的 D 环区的 5 个 SNP 进行了分型,这些母鸡包括 233 只白来航鸡(WL)、50 只 Dekalb-TX35(D-TX)、140 只 barred Plymouth Rock(BPR)和 133 只 Rhode Island Red(RIR),它们均饲养在日本国立畜产草地研究所(NILGS,筑波)。通过 AS-PCR,在这些鸡中观察到了 5 种单倍型。WL、D-TX、BPR 和 RIR 分别显示出 3、2、1 和 4 种 SNP 单倍型。通过 AS-PCR 单倍型与品种的组合,这些鸡被分为 10 组。在每组的两只鸡(20 只个体)中对 D 环进行测序后,观察到了 15 个 SNP 位点(包括一个插入)和 8 个序列单倍型。总之,在 NILGS 的蛋鸡品种中观察到了单倍型变异。本研究表明,mtDNA 中的 SNP 单倍型应该适合推断鸡肉的起源。

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Asian-Australas J Anim Sci. 2013 Feb;26(2):157-62. doi: 10.5713/ajas.2012.12459.