Yang Yan, Li Xiao-Qing, Huang Qing, Huang Shi-Ang
Center for Stem Cell Research and Application, Tongji Medical College Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Jun;19(3):594-7.
In order to investigate the effect of PP2A activator and PP2A inhibitor on proliferation of HL-60 cells and analyze the changes of PP2A activity in patients with acute myeloid leukemia (AML), HL-60 cells were treated with FTY720 alone or in combination with okadaic acid (OA) for 24 hours in culture. Cell proliferation was assayed with CCK8 kit. In addition, 20 AML patients including de novo AML and relapsed AML were enrolled in this study. The activity of PP2A in the peripheral blood mononuclear cells of patients was assayed with a PP2A Immunoprecipitation Phosphatase Assay Kit, the data were analyzed by software SPSS 16.0. The results indicated that as compared with control group, the proliferation of cells in FTY720 group was obviously inhibited (p < 0.05). The proliferation of cells in FTY720 + OA group was slightly inhibited as compared with the control group, there was no statistical difference (p > 0.05), but there was significant difference between the FTY720 + OA and FTY720 groups (p < 0.05). The activity of PP2A in AML patients (453.67 ± 102.52 pmol phosphate) was obviously lower than that in the normal controls (673.29 ± 96.32 pmol phosphate), there was significant difference between them (p < 0.01). It is concluded that the activation or inhibition of PP2A can affect the proliferation of HL-60 cells in vitro. Compared with healthy individuals, the activity of PP2A in AML patients is obviously lower. PP2A protein playing a key role in the occurrence and development of AML may be valuable for the diagnosis and treatment of AML.
为了研究PP2A激活剂和PP2A抑制剂对HL-60细胞增殖的影响，并分析急性髓系白血病（AML）患者PP2A活性的变化，将HL-60细胞在培养中单独用FTY720或与冈田酸（OA）联合处理24小时。用CCK8试剂盒检测细胞增殖。此外，本研究纳入了20例包括初发AML和复发AML的患者。用PP2A免疫沉淀磷酸酶检测试剂盒检测患者外周血单个核细胞中PP2A的活性，数据用SPSS 16.0软件进行分析。结果表明，与对照组相比，FTY720组细胞增殖明显受到抑制（p<0.05）。FTY720+OA组细胞增殖与对照组相比略有抑制，无统计学差异（p>0.05），但FTY720+OA组与FTY720组之间有显著差异（p<0.05）。AML患者PP2A活性（453.67±102.52 pmol磷酸盐）明显低于正常对照组（673.29±96.32 pmol磷酸盐），两者之间有显著差异（p<0.01）。结论是，PP2A的激活或抑制可在体外影响HL-60细胞的增殖。与健康个体相比，AML患者PP2A活性明显降低。PP2A蛋白在AML的发生发展中起关键作用，可能对AML的诊断和治疗具有重要价值。
