Wang Hai-Li, We Wu, Ji Ai-Fan, Shen Xu-Liang, Zhang Guo-Xiang, Zhang Mei-Xiang, Zhai Chun-Yan
First Clinical Department, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Jun;19(3):630-3.
To investigate the apoptosis-induction effect of brucine on human chronic myeloid leukemia cell line K562 cells, K562 cells were exposed to various dosages of brucine. MTT method was used to assayed the growth inhibition effect of brucine on K562 cells. The apoptosis of K562 cells was detected by acridine orange/ethidium bromide (AO/EB) double staining, Annexin-V/PI double labeling method and DNA agarose gel electrophoresis. The results showed that brucine could remarkably inhibit the K562 cell growth in a concentration-dependent and time-dependent manners at the range of 50 to 400 µg/ml, and its most significant inhibition was observed at 400 µg/ml for 72 hours and the inhibition rate was 94.0%. Staining of cells with AO-EB revealed that brucine induced nuclear chromatin condensation. After the K562 cells were treated with the brucine of 400 µg/ml for 72 hours, the most of the nucleus were orange stained and condensation-like or bead-like showing apoptotic morphology. The K562 cells treated with brucine of different concentrations (50, 100, 200, 400, 800 µg/ml) for 72 hours, Annexin-V/PI detection showed brucine could induce apoptosis of K562 cells, and apoptosis rate increased gradually with increasing concentration of drugs. The K562 cells treated with brucine of 400 µg/ml for 72 hours displayed typical ladder strap in DNA gel electrophoresis. It is concluded that brucine can efficiently inhibit cell growth and induce apoptosis of K562 cells with dose-dependent manner in concentrations of 50 - 400 µg/ml.
为研究马钱子碱对人慢性髓系白血病细胞系K562细胞的诱导凋亡作用，将K562细胞暴露于不同剂量的马钱子碱中。采用MTT法检测马钱子碱对K562细胞的生长抑制作用。通过吖啶橙/溴化乙锭（AO/EB）双染、Annexin-V/PI双标记法及DNA琼脂糖凝胶电泳检测K562细胞的凋亡情况。结果表明，在50至400μg/ml范围内，马钱子碱能以浓度和时间依赖性方式显著抑制K562细胞生长，在400μg/ml作用72小时时抑制作用最为显著，抑制率为94.0%。AO-EB细胞染色显示马钱子碱诱导细胞核染色质凝聚。K562细胞经400μg/ml马钱子碱处理72小时后，大部分细胞核呈橙色染色，呈现凝聚状或珠状的凋亡形态。K562细胞经不同浓度（50、100、200、400、800μg/ml）马钱子碱处理72小时后，Annexin-V/PI检测显示马钱子碱可诱导K562细胞凋亡，且凋亡率随药物浓度增加而逐渐升高。K562细胞经400μg/ml马钱子碱处理72小时后，DNA凝胶电泳显示出典型的梯状条带。结论为，在50 - 400μg/ml浓度范围内，马钱子碱能有效抑制K562细胞生长并诱导其凋亡，且呈剂量依赖性。
