Xu Shu-Mei, Zhou Lei, Liu Zhuo-Gang, Chen Bo, Li Yang
Department of Hematology, Chinese Medical University Shengjing Hospital, Shengyang 110021, Liaoning Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Jun;19(3):652-5.
This study was aimed to investigate the effects of glycyrrhetinic acid (GA) on proliferation, apoptosis and survivin mRNA expression in human myeloma cell line U266 in vitro. Cell proliferation was assayed by MTT method. Both cell apoptosis and cell distribution in cell cycle were analyzed by using flow cytometry. Scanning electron microscopy was used to observe the morphological changes in U266 cells induced by GA. Expression of survivin mRNA was detected by quantitative real-time reverse transcription-polymerase chain reaction. The results showed that GA inhibited proliferation of U266 cells in a time- and dose-dependent manners in vitro. GA presented apoptosis induction potency to U266 cells, obvious changes in morphology of U266 cells was observed under scanning electron microscope. The cells were arrested at the G(0)/G(1) phase, showing the accumulation in G(0)/G(1) phase, reduction of cells in G(2)/M phase and S phase. GA could down-regulate the expression of survivin gene in U266 cells in a dose-dependent manner. It is concluded that GA can inhibit proliferation of U266 cells in a time- and dose-dependent manners and induce apoptosis of this cell line in vitro through arresting G(0)/G(1) phase and down-regulating expression of survivin.
本研究旨在探讨甘草次酸（GA）对人骨髓瘤细胞系U266体外增殖、凋亡及生存素mRNA表达的影响。采用MTT法检测细胞增殖。运用流式细胞术分析细胞凋亡及细胞周期分布。通过扫描电子显微镜观察GA诱导的U266细胞形态变化。采用定量实时逆转录-聚合酶链反应检测生存素mRNA的表达。结果表明，GA在体外以时间和剂量依赖性方式抑制U266细胞增殖。GA对U266细胞具有诱导凋亡的作用，扫描电子显微镜下观察到U266细胞形态有明显变化。细胞停滞于G(0)/G(1)期，表现为G(0)/G(1)期细胞蓄积，G(2)/M期和S期细胞减少。GA能以剂量依赖性方式下调U266细胞中生存素基因的表达。结论为，GA可在体外以时间和剂量依赖性方式抑制U266细胞增殖，并通过使细胞停滞于G(0)/G(1)期及下调生存素表达诱导该细胞系凋亡。
