[Th17细胞在小鼠急性移植物抗宿主病早期发病中的作用]

[The role of Th17 cells in early onset of mice acute graft versus host disease].

作者信息

Cheng Hai, Zeng Ling-yu, Pan Bin, Song Guo-liang, Tian Jing, Chen Chong, Yan Zhi-ling, Li Zhen-yu, Xu Kai-lin

机构信息

Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2011 May;32(5):322-5.

PMID:21729601

Abstract

OBJECTIVE

To explore the role of Th17 cells in early onset of acute graft-versus-host disease (aGVHD) and its mechanism.

METHODS

Mice aGVHD model was established by irradiated BABL/c mice inoculated with mixed suspension of C57BL/6 bone marrow cells and splenocytes. The mice were divided into 4 groups: (1) normal control, (2) irradiated group, (3) allo-BMT + DMSO group, (4) allo-BMT + halofuginone (HF) group. HF was given intraperitoneally at 5 µg per mouse from -1 d to +10 d after allogeneic bone marrow transplantation(allo-BMT).Mice aGVHD symptoms and survival were observed. Th1/Th17 cells ratio was evaluated by flow cytometry.

RESULTS

All the experimental groups (3) and (4) developed aGVHD after transplantation. More severe aGVHD was observed in group (4) than in group (3). HF prevented cutaneous aGVHD in all the mice, but augmented hepatic and small intestine GVHD. The percentage of Th17 cells and the ratio of Th1/Th17 were significantly higher while the percentage of Th1 cells was significantly lower in group (4) at day +6 (P < 0.05).

CONCLUSION

Early blockage of Th17 cell results in increase of Th1 cell percentage, which exacerbates aGVHD.

摘要

目的

探讨Th17细胞在急性移植物抗宿主病（aGVHD）早期发病中的作用及其机制。

方法

通过给经照射的BABL/c小鼠接种C57BL/6骨髓细胞和脾细胞的混合悬液建立小鼠aGVHD模型。将小鼠分为4组：（1）正常对照组，（2）照射组，（3）异基因骨髓移植+二甲基亚砜组，（4）异基因骨髓移植+常山酮（HF）组。在异基因骨髓移植（allo-BMT）后-1天至+10天，每只小鼠腹腔注射5μg HF。观察小鼠aGVHD症状和生存情况。通过流式细胞术评估Th1/Th17细胞比例。