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一种去交换三重突变牛气味结合蛋白的表征

Characterization of a deswapped triple mutant bovine odorant binding protein.

作者信息

Polverini Eugenia, Lardi Paolo, Mazzini Alberto, Sorbi Robert T, Virna Conti, Ramoni Roberto, Favilla Roberto

机构信息

Department of Physics and CNISM, University of Parma, V.le Usberti 7A, Parma, Italy; E-Mails:

出版信息

Int J Mol Sci. 2011;12(4):2294-314. doi: 10.3390/ijms12042294. Epub 2011 Apr 4.

DOI:10.3390/ijms12042294
PMID:21731442
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3127118/
Abstract

The stability and functionality of GCC-bOBP, a monomeric triple mutant of bovine odorant binding protein, was investigated, in the presence of denaturant and in acidic pH conditions, by both protein and 1-aminoanthracene ligand fluorescence measurements, and compared to that of both bovine and porcine wild type homologues. Complete reversibility of unfolding was observed, though refolding was characterized by hysteresis. Molecular dynamics simulations, performed to detect possible structural changes of the monomeric scaffold related to the presence of the ligand, pointed out the stability of the β-barrel lipocalin scaffold.

摘要

通过蛋白质和1-氨基蒽配体荧光测量，研究了牛气味结合蛋白的单体三重突变体GCC-bOBP在变性剂存在和酸性pH条件下的稳定性和功能，并与牛和猪的野生型同源物进行了比较。观察到去折叠的完全可逆性，尽管复性表现出滞后现象。进行分子动力学模拟以检测与配体存在相关的单体支架可能的结构变化，结果表明β-桶状脂质运载蛋白支架的稳定性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fc/3127118/53d9922a600c/ijms-12-02294f9.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fc/3127118/daa138a154dc/ijms-12-02294f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fc/3127118/02a9540ba63d/ijms-12-02294f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fc/3127118/457d3e4e8bac/ijms-12-02294f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fc/3127118/d6859bea15ad/ijms-12-02294f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fc/3127118/53d9922a600c/ijms-12-02294f9.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fc/3127118/5c0e26d1a53e/ijms-12-02294f2.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fc/3127118/eb2fa50c3f4e/ijms-12-02294f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fc/3127118/daa138a154dc/ijms-12-02294f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fc/3127118/02a9540ba63d/ijms-12-02294f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fc/3127118/457d3e4e8bac/ijms-12-02294f7.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3fc/3127118/53d9922a600c/ijms-12-02294f9.jpg

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本文引用的文献

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PLoS One. 2010 Nov 30;5(11):e15035. doi: 10.1371/journal.pone.0015035.
2
Phenylalanine 35 and tyrosine 82 are involved in the uptake and release of ligand by porcine odorant-binding protein.苯丙氨酸35和酪氨酸82参与猪嗅觉结合蛋白对配体的摄取和释放。
Biochim Biophys Acta. 2009 Aug;1794(8):1142-50. doi: 10.1016/j.bbapap.2009.04.012. Epub 2009 May 3.
3
Wild-type and mutant bovine odorant-binding proteins to probe the role of the quaternary structure organization in the protein thermal stability.
野生型和突变型牛气味结合蛋白，以探究四级结构组织在蛋白质热稳定性中的作用。
J Proteome Res. 2008 Dec;7(12):5221-9. doi: 10.1021/pr800528b.
4
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Proteins. 2008 Aug;72(2):769-78. doi: 10.1002/prot.21966.
5
The tryptophan phosphorescence of porcine and mutant bovine odorant-binding proteins: a probe for the local protein structure and dynamics.猪和突变牛气味结合蛋白的色氨酸磷光：一种用于探测局部蛋白质结构和动力学的探针。
J Proteome Res. 2008 Mar;7(3):1151-8. doi: 10.1021/pr700755z. Epub 2008 Jan 31.
6
Dissociation and unfolding of bovine odorant binding protein at acidic pH.牛气味结合蛋白在酸性pH条件下的解离与去折叠
J Struct Biol. 2007 Jul;159(1):82-91. doi: 10.1016/j.jsb.2007.02.007. Epub 2007 Mar 4.
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8
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9
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J Comput Chem. 2005 Dec;26(16):1701-18. doi: 10.1002/jcc.20291.
10
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Biochim Biophys Acta. 2005 Jun 15;1750(1):30-9. doi: 10.1016/j.bbapap.2005.04.003. Epub 2005 Apr 20.