Department of Chemical Engineering, University of São Paulo, São Paulo, SP, Brazil.
Antonie Van Leeuwenhoek. 2011 Nov;100(4):619-30. doi: 10.1007/s10482-011-9617-7. Epub 2011 Jul 6.
The Kluyveromyces marxianus strains CBS 6556, CBS 397 and CBS 712(T) were cultivated on a defined medium with either glucose, lactose or sucrose as the sole carbon source, at 30 and 37°C. The aim of this work was to evaluate the diversity within this species, in terms of the macroscopic physiology. The main properties evaluated were: intensity of the Crabtree effect, specific growth rate, biomass yield on substrate, metabolite excretion and protein secretion capacity, inferred by measuring extracellular inulinase activity. The strain Kluyveromyces lactis CBS 2359 was evaluated in parallel, since it is the best described Kluyveromyces yeast and thus can be used as a control for the experimental setup. K. marxianus CBS 6556 presented the highest specific growth rate (0.70 h(-1)) and the highest specific inulinase activity (1.65 U mg(-1) dry cell weight) among all strains investigated, when grown at 37°C with sucrose as the sole carbon source. The lowest metabolite formation and highest biomass yield on substrate (0.59 g dry cell weight g sucrose(-1)) was achieved by K. marxianus CBS 712(T) at 37°C. Taken together, the results show a systematic comparison of carbon and energy metabolism among three of the best known K. marxianus strains, in parallel to K. lactis CBS 2359.
马克斯克鲁维酵母 CBS 6556、CBS 397 和 CBS 712(T)菌株在以葡萄糖、乳糖或蔗糖为唯一碳源的限定培养基中，于 30 和 37°C 下进行培养。本工作旨在评估该物种在宏观生理学方面的多样性。评估的主要特性包括：Crabtree 效应强度、比生长速率、基质上的生物量产量、代谢物排泄和蛋白质分泌能力，通过测量细胞外菊粉酶活性来推断。同时评估了乳糖克鲁维酵母 CBS 2359 菌株，因为它是描述最详细的克鲁维酵母，因此可用作实验装置的对照。当以蔗糖为唯一碳源在 37°C 下生长时，马克斯克鲁维酵母 CBS 6556 表现出最高的比生长速率（0.70 h(-1))和最高的比菊粉酶活性（1.65 U mg(-1)干细胞重量）。在 37°C 下，马克斯克鲁维酵母 CBS 712(T) 的代谢物形成最低，基质上的生物量产量最高（0.59 g 干细胞重量 g 蔗糖(-1))。综合来看，这些结果显示了对三种最知名的马克斯克鲁维酵母菌株（与乳糖克鲁维酵母 CBS 2359 平行）的碳和能量代谢进行了系统比较。
