School of Plant, Environmental, and Soil Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
Mol Biotechnol. 2012 May;51(1):18-26. doi: 10.1007/s12033-011-9436-0.
Petroleum hydrocarbons (PHC) in soil are potentially toxic to plants and exert negative effect on the environment and human health. To understand the effect of PHC on the gene expression profile of a wetland plant Spartina alterniflora in the coastal Louisiana, plants were subject up to 40% PHC under greenhouse conditions. The plants exposed to PHC showed 21% reduction of leaf total chlorophyll after 2 weeks of stress. Using 20 annealing control primers, 28 differentially expressing genes (DEGs) were identified in leaf and root tissues of S. alterniflora in response to PHC stress. Eleven of these 28 DEGs had role in either molecular function (chlorophyll a-b binding protein, HSP70, NADH, RAN1-binding protein, and RNA-binding protein), biological processes (cell wall protein, nucelosome/chromatin assembly factor) or cellular function (30 S ribosomal protein). This indicated that genes in different regulatory pathways of S. alterniflora were involved in response to PHC. All DEGs showed reduced transcript accumulation in root under oil stress, whereas they showed up- or down-regulation in their transcript abundance in leaf depending on the concentration of the PHC. The genes identified through this study could be used in the genetic screen of S. alterniflora for resistance to PHC.
土壤中的石油烃（PHC）对植物具有潜在毒性，并对环境和人类健康产生负面影响。为了了解 PHC 对路易斯安那州沿海湿地植物互花米草基因表达谱的影响，在温室条件下，植物受到高达 40%的 PHC 暴露。在受到 PHC 胁迫 2 周后，暴露于 PHC 中的植物的叶片总叶绿素减少了 21%。使用 20 个退火对照引物，在响应 PHC 胁迫的互花米草叶片和根组织中鉴定出 28 个差异表达基因（DEGs）。这 28 个 DEGs 中的 11 个在分子功能（叶绿素 a-b 结合蛋白、HSP70、NADH、RAN1 结合蛋白和 RNA 结合蛋白）、生物过程（细胞壁蛋白、核小体/染色质组装因子）或细胞功能（30S 核糖体蛋白）中发挥作用。这表明互花米草不同调控途径的基因参与了对 PHC 的响应。所有 DEGs 在根中表现出在油胁迫下的转录积累减少，而在叶片中根据 PHC 的浓度表现出上调或下调的转录丰度。通过这项研究鉴定的基因可用于互花米草对 PHC 抗性的遗传筛选。
