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Setting up and running an advanced light microscopy and imaging facility.

作者信息

Sánchez Carlos, Muñoz Ma Ángeles, Villalba Maite, Labrador Verónica, Díez-Guerra F Javier

机构信息

Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Madrid, Spain.

出版信息

Curr Protoc Cytom. 2011 Jul;Chapter 12:Unit 12.22. doi: 10.1002/0471142956.cy1222s57.

DOI:10.1002/0471142956.cy1222s57
PMID:21732308
Abstract

During the last twenty years, interest in light microscopy and imaging techniques has grown in various fields, such as molecular and cellular biology, developmental biology, and neurobiology. In addition, the number of scientific articles and journals using these techniques is rapidly increasing. Nowadays, most research institutions require sophisticated microscopy systems to cover their investigation demands. In general, such instruments are too expensive and complex to be purchased and managed by a single laboratory or research group, so they have to be shared with other groups and supervised by specialized personnel. This is the reason why microscopy and imaging facilities are becoming so important at research institutions nowadays. In this unit, we have gathered and presented a number of issues and considerations from our own experience that we hope will be helpful when planning or setting up a new facility.

摘要

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