通过将来自一名ABO血型不相合骨髓移植患者的爱泼斯坦-巴尔病毒转化B淋巴细胞与小鼠骨髓瘤细胞构建的杂交瘤产生的血型糖基转移酶单克隆抗体。
Monoclonal antibody to blood group glycosyltransferases, produced by hybrids constructed with Epstein-Barr-virus-transformed B lymphocytes from a patient with ABO-incompatible bone marrow transplant and mouse myeloma cells.
作者信息
Kominato Y, Fujikura T, Shimada I, Takizawa H, Hayashi K, Mori T, Matsuda T
机构信息
Department of Legal Medicine, Toyama Medical and Pharmaceutical University, Japan.
出版信息
Vox Sang. 1990;59(2):116-8. doi: 10.1111/j.1423-0410.1990.tb05022.x.
A patient with chronic myeloid leukemia secreted an antibody to blood group glycosyltransferases after ABO-incompatible bone marrow transplantation (B recipient/O donor). Peripheral B lymphocytes from the recipient were transformed with Epstein-Barr virus, and then fused by polyethylene glycol with mouse myeloma cell line P3-X63/Ag8.653. After the cloning of the hybridoma cells, a cell line which produced human IgM antibody to blood group glycosyltransferases was established. The antibody completely neutralized B transferase activity at low concentration, while a larger amount of immunoglobulins was required to neutralize A transferase activity.
一名慢性髓性白血病患者在ABO血型不相合的骨髓移植（B型受者/O型供者）后分泌了一种针对血型糖基转移酶的抗体。将受者的外周B淋巴细胞用爱泼斯坦-巴尔病毒转化，然后用聚乙二醇与小鼠骨髓瘤细胞系P3-X63/Ag8.653融合。在对杂交瘤细胞进行克隆后，建立了一种产生针对血型糖基转移酶的人IgM抗体的细胞系。该抗体在低浓度时能完全中和B转移酶活性，而中和A转移酶活性则需要大量的免疫球蛋白。
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