Department of Pediatrics, Seoul National University, Boramae Medical Center, Seoul, Republic of Korea.
J Pediatr Gastroenterol Nutr. 2011 Nov;53(5):512-9. doi: 10.1097/MPG.0b013e318227e518.
The aim of the study was to investigate the previously unexplored diversity of neonatal intestinal microbiota and monitor early intestinal colonization patterns in Korean preterm infants using high-throughput pyrosequencing technology combined with 16S rDNA-based molecular methods.
A total of 46,369 partial 16S rDNA sequences obtained from 30 fecal samples serially taken from 10 very-low-birth weight preterm infants were analyzed.
A significant proportion of the molecular species (21.9%) was found to be unclassified. The pathogenic or potentially pathogenic molecular species belonging to the classes Gammaproteobacteria and Bacilli were predominant during the entire observation period. Anaerobic or nonpathogenic molecular species belonging to the class Clostridia (except Clostridium difficile) and phyla Bacteroidetes were ubiquitous even within 72 hours after birth. The proportion of these species increased significantly at 1 month of age. The most ubiquitous and abundant major molecular genera common to all of the postnatal ages were Escherichia, Enterobacter, Enterococcus, Veillonella, Serratia, Staphylococcus, Roseburia, Acinetobacter, Citrobacter, Bacteroides, Faecalibacterium, Blautia, and Streptococcus.
The diversity and dynamic nature of intestinal bacterial colonization in very-low-birth weight preterm infants were revealed using pyrosequencing technology. The results of the present pilot study may provide a basis to consider when investigating or interpreting the role of intestinal microbiota in certain preterm infant diseases such as necrotizing enterocolitis or systemic infection.
本研究旨在通过高通量焦磷酸测序技术结合 16S rDNA 分子方法，探索先前未知的新生儿肠道微生物多样性，并监测韩国早产儿的早期肠道定植模式。
对 10 例极低出生体重早产儿的 30 份粪便样本进行连续采集，共获得 46369 个部分 16S rDNA 序列，并进行分析。
发现相当比例的分子种（21.9%）未分类。在整个观察期间，属于γ变形菌纲和芽孢杆菌纲的致病性或潜在致病性分子种占主导地位。属于梭菌纲（艰难梭菌除外）和拟杆菌门的厌氧或非致病性分子种即使在出生后 72 小时内也普遍存在。这些物种的比例在 1 个月时显著增加。在所有出生后年龄中最普遍和丰富的主要分子属是大肠杆菌、肠杆菌、肠球菌、韦荣球菌、沙雷氏菌、葡萄球菌、罗氏菌、醋酸杆菌、柠檬酸杆菌、拟杆菌、粪杆菌、布劳特氏菌和链球菌。
本研究通过焦磷酸测序技术揭示了极低出生体重早产儿肠道细菌定植的多样性和动态性。本研究的初步结果可为研究或解释肠道微生物群在某些早产儿疾病（如坏死性小肠结肠炎或全身感染）中的作用提供依据。
