链脲佐菌素诱导新生大鼠胰岛α细胞中 Ngn3 和 Pax4 的表达。
Streptozotocin-induced expression of Ngn3 and Pax4 in neonatal rat pancreatic α-cells.
机构信息
Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
出版信息
World J Gastroenterol. 2011 Jun 21;17(23):2812-20. doi: 10.3748/wjg.v17.i23.2812.
AIM
To investigate the mechanism behind β-cell regeneration in neonatal rat pancreas treated with streptozotocin (STZ).
METHODS
Neonatal Sprague Dawley rats were intraperitoneally injected with 70 mg/kg STZ. Body weight, pancreas weight and blood glucose were recorded every two days after the treatment. To identify the expression and location of transcription factors in the rat pancreas, double immunofluorescent staining was performed using antibodies to specific cell markers and transcription factors.
RESULTS
Expression of Neurogenin 3 (Ngn3), a marker for endocrine precursor cells, was observed by immunofluorescence in a few β-cells and many α-cells. The expression reached a peak 12 d after treatment. Pax4, a transcription factor that lies downstream of Ngn3 and plays an important role in β-cell differentiation, was also expressed in the α-cells of STZ-treated rats. We did not observe significant changes in Nkx6.1, which is essential for β-cell maturation in the treated rats.
CONCLUSION
α-cells dedifferentiated into endocrine precursor cells and acquired the ability to dedifferentiate in the neonatal rat pancreas after STZ treatment.
目的
研究链脲佐菌素（STZ）处理新生大鼠胰腺β细胞再生的机制。
方法
新生 Sprague Dawley 大鼠腹腔内注射 70mg/kg STZ。治疗后每两天记录一次体重、胰腺重量和血糖。为了鉴定大鼠胰腺中转录因子的表达和位置，使用特定细胞标志物和转录因子的抗体进行双重免疫荧光染色。
结果
免疫荧光观察到神经基因 3（Ngn3），一种内分泌前体细胞的标志物，在少数β细胞和许多α细胞中表达。治疗后 12 天达到峰值。Pax4 是 Ngn3 下游的转录因子，在 STZ 处理大鼠的α细胞中也有表达，在β细胞分化中起重要作用。我们没有观察到 Nkx6.1 有明显变化，它是β细胞成熟所必需的。
结论
STZ 处理后，新生大鼠胰腺中的α细胞去分化为内分泌前体细胞，并获得了去分化的能力。
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