间皮素在新生大鼠胰腺重塑过程中促进细胞增殖。
Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas.
作者信息
Yin Dan-Dan, You Liang-Hui, Yuan Qing-Xin, Liang Xiao-Di, Wang Ning, Wang Lin-Tao, Yuan Li, Wang Ke-Ming, De Wei
机构信息
Dan-Dan Yin, Department of Central Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, Jiangsu Province, China.
出版信息
World J Gastroenterol. 2014 Jun 14;20(22):6884-96. doi: 10.3748/wjg.v20.i22.6884.
AIM
To investigate the effect of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.
METHODS
Overexpression or downregulation of mesothelin expression in INS-1 cells was carried out to investigate the effect of mesothelin during cell proliferation and cell apoptosis in vitro. Adenovirus-mediated RNA interference was performed to block mesothelin in vivo to directly assess the role of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.
RESULTS
Exogenous overexpression of mesothelin promoted cell proliferation, cell colony formation and enhanced cell resistance to apoptosis of INS-1 cells. Down-regulation of mesothelin made no difference in cell proliferation and apoptosis compared with that in the control group. After an injection of adenovirus-mesothelin, a significantly increased number of small islets appeared, and the expression of PCNA was decreased on day 7 and day 14 compared with the Ad-EGFP group.
CONCLUSION
Mesothelin was able to promote β cell proliferation in the remodeling stage of neonatal rats. Mesothelin may have an important role in the remodeling of the endocrine pancreas in neonatal rats.
目的
研究间皮素在新生大鼠内分泌胰腺重塑中的作用。
方法
在INS-1细胞中进行间皮素表达的过表达或下调，以研究间皮素在体外细胞增殖和细胞凋亡过程中的作用。采用腺病毒介导的RNA干扰在体内阻断间皮素，以直接评估间皮素在新生大鼠内分泌胰腺重塑中的作用。
结果
间皮素的外源性过表达促进了INS-1细胞的增殖、细胞集落形成，并增强了细胞对凋亡的抵抗能力。与对照组相比，间皮素的下调对细胞增殖和凋亡没有影响。注射腺病毒-间皮素后，出现的小胰岛数量显著增加，与Ad-EGFP组相比，在第7天和第14天PCNA的表达降低。
结论
间皮素能够促进新生大鼠重塑阶段的β细胞增殖。间皮素可能在新生大鼠内分泌胰腺的重塑中起重要作用。
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