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成年和新生大鼠心脏中ADP-核糖基化产物的定位及部分特性研究

Localization and partial characterization of ADP-ribosylation products in hearts from adult and neonatal rats.

作者信息

Piron K J, McMahon K K

机构信息

Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock 79430.

出版信息

Biochem J. 1990 Sep 15;270(3):591-7. doi: 10.1042/bj2700591.

DOI:10.1042/bj2700591
PMID:2173547
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1131773/
Abstract

The subcellular distributions of endogenous ADP-ribosylation products in hearts from 1-day-old neonatal and adult rats were investigated. In adult rat heart a 52 kDa mono-ADP-ribosylation product was identified in the plasma membrane fraction. In contrast, in neonatal rat heart a 130 kDa poly-ADP-ribosylation product was present in the nuclear fraction. The monomeric and polymeric nature of the two ADP-ribosylation products was determined by their sensitivity to thymidine and by analysis of their snake venom phosphodiesterase products. NADP+ enhanced both the mono- and polymeric reactions. The ADP-ribose-protein linkage of the adult 52 kDa product was stable to 1 h of treatment with hydroxylamine (0.5 M) and mercury ions, but was sensitive to alkali and a 12 h treatment with hydroxylamine (1 M). This is suggestive of an arginine linkage. The 130 kDa poly-ADP-ribosylation product from the neonatal rat heart was alkalilabile but stable to both hydroxylamine and HgCl2. This implies the presence of an unusual linkage in the 130 kDa product. The presence of these different ADP-ribosylation products in adult and neonatal rat hearts suggests the possible importance of these proteins and their ADP-ribosylation during cardiac development.

摘要

研究了1日龄新生大鼠和成年大鼠心脏中内源性ADP-核糖基化产物的亚细胞分布。在成年大鼠心脏中，在质膜部分鉴定出一种52 kDa的单ADP-核糖基化产物。相反，在新生大鼠心脏中，一种130 kDa的多ADP-核糖基化产物存在于核部分。通过它们对胸苷的敏感性以及对其蛇毒磷酸二酯酶产物的分析，确定了两种ADP-核糖基化产物的单体和聚合物性质。NADP+增强了单反应和聚合反应。成年52 kDa产物的ADP-核糖-蛋白质连接对用羟胺（0.5 M）和汞离子处理1小时稳定，但对碱和用羟胺（1 M）处理12小时敏感。这提示是精氨酸连接。新生大鼠心脏的130 kDa多ADP-核糖基化产物对碱不稳定，但对羟胺和HgCl2均稳定。这意味着在130 kDa产物中存在不寻常的连接。成年和新生大鼠心脏中这些不同的ADP-核糖基化产物的存在表明这些蛋白质及其ADP-核糖基化在心脏发育过程中可能具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/c75f4c5caf0b/biochemj00175-0040-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/51131a479b0d/biochemj00175-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/aac3c7782e63/biochemj00175-0038-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/a0065887fff6/biochemj00175-0038-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/ec349eea9637/biochemj00175-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/caed44006d45/biochemj00175-0039-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/59d6bf57e8d7/biochemj00175-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/c75f4c5caf0b/biochemj00175-0040-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/51131a479b0d/biochemj00175-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/aac3c7782e63/biochemj00175-0038-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/a0065887fff6/biochemj00175-0038-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/ec349eea9637/biochemj00175-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/caed44006d45/biochemj00175-0039-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/59d6bf57e8d7/biochemj00175-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc69/1131773/c75f4c5caf0b/biochemj00175-0040-b.jpg

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本文引用的文献

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Gs alpha is a substrate for mono(ADP-ribosyl)transferase of NG108-15 cells. ADP-ribosylation regulates Gs alpha activity and abundance.Gsα是NG108-15细胞单（ADP-核糖基）转移酶的底物。ADP-核糖基化调节Gsα的活性和丰度。
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The effect of in vivo treatment with triiodothyronine on the in vitro synthesis of protein-poly(ADP)-ribose adducts by isolated cardiocyte nuclei and the separation of poly(ADP)-ribosylated proteins by phenol extraction and electrophoresis.
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