Yu Chun-Mei, Zhang Ping, Wang Jing, Liu Ming-Xi, Wang Hui, Zhou Zuo-Min, Sha Jia-Hao
Jiangsu Provincial Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Zhonghua Nan Ke Xue. 2011 Jun;17(6):516-22.
To study the expression of Annexin A7 in the mouse testis, especially in different types of spermatogonia.
We prepared Annexin A7 recombinant protein using prokaryotic expression, adsorbed the Annexin A7 antibody with it after identified by mass spectrometry, and detected the expression of Annexin A7 by Western-blot and immunohistochemistry.
Annexin A7 was expressed in a development-dependent manner in the spermatogonia of the prepubertal mice and in the type-A single (As) and type-A paired (Apr) spermatogonia of adult mice. These results were confirmed by the co-localization of Annexin A7 and Stra8, a known determinant of differentiated spermatogonial stem cells (SSCs).
Annexin A7 is the internal factor of As and Apr spermatogonia, which might be involved in the biological functions of SSCs.
研究膜联蛋白A7在小鼠睾丸中的表达，尤其是在不同类型精原细胞中的表达。
我们利用原核表达制备膜联蛋白A7重组蛋白，经质谱鉴定后用其吸附膜联蛋白A7抗体，并用蛋白质免疫印迹法和免疫组织化学法检测膜联蛋白A7的表达。
膜联蛋白A7在青春期前小鼠的精原细胞以及成年小鼠的A型单倍体（As）和A型配对体（Apr）精原细胞中呈发育依赖性表达。膜联蛋白A7与Stra8（一种已知的分化精原干细胞决定因子）的共定位证实了这些结果。
膜联蛋白A7是As和Apr精原细胞的内在因子，可能参与精原干细胞的生物学功能。
