[Expression of annexin A7 in spermatogonial stem cells].

OBJECTIVE

To study the expression of Annexin A7 in the mouse testis, especially in different types of spermatogonia.

METHODS

We prepared Annexin A7 recombinant protein using prokaryotic expression, adsorbed the Annexin A7 antibody with it after identified by mass spectrometry, and detected the expression of Annexin A7 by Western-blot and immunohistochemistry.

RESULTS

Annexin A7 was expressed in a development-dependent manner in the spermatogonia of the prepubertal mice and in the type-A single (As) and type-A paired (Apr) spermatogonia of adult mice. These results were confirmed by the co-localization of Annexin A7 and Stra8, a known determinant of differentiated spermatogonial stem cells (SSCs).

CONCLUSION

Annexin A7 is the internal factor of As and Apr spermatogonia, which might be involved in the biological functions of SSCs.