Narimatsu S, Akutsu Y, Matsunaga T, Watanabe K, Yamamoto I, Yoshimura H
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Biochem Biophys Res Commun. 1990 Oct 30;172(2):607-13. doi: 10.1016/0006-291x(90)90717-2.
An isozyme of cytochrome P450 was purified from liver microsomes of guinea pigs by HPLC with anion-exchange and hydroxylapatite columns. The isozyme showed a single band of 52 kdalton on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Oxidation activities of the final preparation towards p-nitroanisole, aniline and d-benzphetamine were 1.3 to 15.4-fold comparing with those of microsomal fraction. This isozyme was also concerned with oxidation of delta 9-tetrahydrocannabinol (THC) to 8 alpha-hydroxy- (8 alpha-OH-), 2'-OH- and 3'-OH-delta 9-THCs. The N-terminal region of the isozyme was considerably hydrophobic, and 13 of the first 20 amino acid residues was leucine. Since this first 20 amino acid sequence is 80% homologous with that of cytochrome P450 LM2 purified from rabbit liver microsomes, this isozyme can be categorized to a subfamily of P450 IIB.
通过使用阴离子交换柱和羟基磷灰石柱的高效液相色谱法，从豚鼠肝脏微粒体中纯化出一种细胞色素P450同工酶。该同工酶在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上显示出一条52千道尔顿的单条带。最终制剂对对硝基苯甲醚、苯胺和d-苄非他明的氧化活性与微粒体部分相比为1.3至15.4倍。这种同工酶还参与将δ9-四氢大麻酚（THC）氧化为8α-羟基-（8α-OH-）、2'-OH-和3'-OH-δ9-THC。该同工酶的N端区域具有相当大的疏水性，前20个氨基酸残基中有13个是亮氨酸。由于这前20个氨基酸序列与从兔肝脏微粒体中纯化的细胞色素P450 LM2的序列有80%的同源性，这种同工酶可归类为P450 IIB亚家族。
