Laboratory of Nuclear Techniques, Veterinary Faculty, University of Uruguay, Lasplaces 1550, 11600 Montevideo, Uruguay.
Res Vet Sci. 2012 Aug;93(1):108-13. doi: 10.1016/j.rvsc.2011.06.002. Epub 2011 Jul 6.
Thyrotropin receptor (TSH-R), thyroglobulin (Tg), thyroperoxidase (TPO), thyroid specific transcription factor-1 (TTF-1), paired box 8 transcription factor (PAX-8), insulin like growth factor-1 (IGF-1) and estrogen receptor alpha (ERα) transcripts were determined by real-time PCR in follicular carcinoma and contralateral (CL) lobes, and healthy thyroid canine glands. Concentrations of TSH-R, PAX-8, and ERα mRNA were not different among groups; the carcinoma group had lower Tg and TPO mRNA than healthy and CL groups, while no differences were found between the two latter groups, suggesting that the carcinoma tissue presents an altered capacity to synthesize thyroid hormones. The transcription factor that promotes thyrocytes proliferation, TTF-1 as well as IGF-1, presented a greater mRNA expression in the CL group, suggesting that the CL lobe may function in a compensatory state.
促甲状腺激素受体 (TSH-R)、甲状腺球蛋白 (Tg)、甲状腺过氧化物酶 (TPO)、甲状腺特异性转录因子-1 (TTF-1)、配对盒 8 转录因子 (PAX-8)、胰岛素样生长因子-1 (IGF-1) 和雌激素受体α (ERα) 转录本通过实时 PCR 在滤泡状癌和对侧 (CL) 叶以及健康犬甲状腺中进行了测定。TSH-R、PAX-8 和 ERα mRNA 的浓度在各组之间没有差异；癌组的 Tg 和 TPO mRNA 低于健康组和 CL 组，而后两组之间没有差异，表明癌组织合成甲状腺激素的能力发生了改变。促进甲状腺细胞增殖的转录因子 TTF-1 和 IGF-1 在 CL 组中的 mRNA 表达更高，这表明 CL 叶可能处于代偿状态。
