Department of Internal Medicine and Chronobiology Unit, S. Giovanni Rotondo, Italy.
Biomed Pharmacother. 2011 Jul;65(4):275-9. doi: 10.1016/j.biopha.2011.02.002. Epub 2011 Apr 8.
Any quantity varying in the spatial-temporal dimension may be considered as a signal. Human lymphocyte cell surface molecules and subsets present circadian variation and this variation may represent a kind of signalling in the neuroendocrine-immune system. We have analyzed the dynamics of variation of specific lymphocyte subsets in healthy humans.
In our study, lymphocyte subpopulation analyses were performed and cortisol, melatonin, GH and TSH serum levels were measured on blood samples collected every 4h for 24 hours from eleven healthy men, ages 35-53 years (mean=44±6SD).
A clear circadian rhythm was validated for CD8 and cortisol with acrophase during the day and for CD3, CD4, melatonin, GH and TSH with acrophase at night. Cross-correlation showed that CD3 correlated positively with CD4 (ρ=0.67, P<0.05) and negatively with CD8 (ρ=-0.41, P<0.05), CD4 correlated positively with melatonin (ρ=0.90, P<0.05), GH (ρ=0.92, P<0.05) and TSH (ρ=0.71, P<0.05), negatively with CD8 (ρ=-0.90, P<0.05) and cortisol (ρ=-0.18, P<0.05), CD8 correlated positively with cortisol (ρ=0.38, P<0.05).
The different profiles of nyctohemeral changes of lymphocyte cell surface molecules and specific lymphocyte subsets realize different relationships with the neuroendocrine hormones and might represent a way of signal transmission among the multiple components of the neuroendocrine-immune system.
任何在时空维度上变化的量都可以被视为信号。人类淋巴细胞表面分子和亚群呈现昼夜节律变化，这种变化可能代表神经内分泌-免疫系统中的一种信号传递方式。我们分析了健康人群中特定淋巴细胞亚群变化的动力学。
在我们的研究中，对 11 名年龄在 35-53 岁（平均 44±6SD）的健康男性进行了淋巴细胞亚群分析，并测量了每 4 小时采集一次的 24 小时血液样本中的皮质醇、褪黑素、GH 和 TSH 血清水平。
验证了 CD8 和皮质醇的明显昼夜节律，其峰值出现在白天，而 CD3、CD4、褪黑素、GH 和 TSH 的峰值出现在晚上。交叉相关显示 CD3 与 CD4 呈正相关（ρ=0.67，P<0.05），与 CD8 呈负相关（ρ=-0.41，P<0.05），CD4 与褪黑素（ρ=0.90，P<0.05）、GH（ρ=0.92，P<0.05）和 TSH（ρ=0.71，P<0.05）呈正相关，与 CD8（ρ=-0.90，P<0.05）和皮质醇（ρ=-0.18，P<0.05）呈负相关，CD8 与皮质醇（ρ=0.38，P<0.05）呈正相关。
淋巴细胞表面分子和特定淋巴细胞亚群的昼夜节律变化的不同模式与神经内分泌激素之间存在不同的关系，可能代表了神经内分泌-免疫系统多个组成部分之间信号传递的一种方式。
