Kitamura K, Yamada K, Kuzushima K, Morishima T, Morishima Y, Yamamoto N, Nishiyama Y, Ohya K, Yamaguchi H
Department of Internal Medicine, Branch Hospital, Nagoya, Japan.
J Med Virol. 1990 Sep;32(1):60-6. doi: 10.1002/jmv.1890320111.
Immunoglobulin G class human monoclonal antibodies to human cytomegalovirus (HCMV) were produced by the fusion of Epstein-Barr virus-transformed B cells and a murine myeloma cell line. The B cells were derived from the peripheral blood lymphocytes of healthy adult volunteers. Four hybridomas producing HCMV-specific monoclonal antibodies were established and each of four antibodies immunoprecipitated an HCMV-specific protein with a molecular weight of 68 kDa. However, the antibodies differed in some of their properties as characterized by indirect immunofluorescence assay and immunoblotting studies, due to the detection of different epitopes on the reacting antigen. None of the four antibodies had any virus neutralizing activity.
通过将爱泼斯坦-巴尔病毒转化的B细胞与鼠骨髓瘤细胞系融合，产生了针对人巨细胞病毒（HCMV）的免疫球蛋白G类人单克隆抗体。这些B细胞来源于健康成年志愿者的外周血淋巴细胞。建立了四个产生HCMV特异性单克隆抗体的杂交瘤，四种抗体中的每一种都免疫沉淀了一种分子量为68 kDa的HCMV特异性蛋白。然而，由于在反应抗原上检测到不同的表位，通过间接免疫荧光测定和免疫印迹研究表征，这四种抗体在某些特性上有所不同。这四种抗体均无任何病毒中和活性。
