Flint J S, Ballantyne G H, Goldenring J R, Fielding L P, Modlin I M
St Mary's Hospital, Waterbury, Conn.
Arch Surg. 1990 Dec;125(12):1561-3. doi: 10.1001/archsurg.1990.01410240039008.
We have studied the effect of neuropeptide Y on basal and vasoactive intestinal polypeptide-stimulated changes in the short-circuit current of strips of colonic mucosa from New Zealand white rabbits mounted in Ussing chambers. When administered to the basolateral surface, neuropeptide Y is found to decrease basal short-circuit current. Neuropeptide Y inhibits vasoactive intestinal peptide-stimulated increases in short-circuit current in a concentration-dependent fashion by a tetrodotoxin-insensitive mechanism. Also, neuropeptide Y inhibited increases in short-circuit current produced by direct stimulation of adenylate cyclase with forskolin. Furthermore, neuropeptide Y prevents vasoactive intestinal peptide-stimulated increases in tissue cyclic adenosine monophosphate levels. These results indicate that neuropeptide Y administered to the basolateral membrane inhibits vasoactive intestinal peptide-stimulated short-circuit current changes by a tetrodotoxin-insensitive mechanism that decreases tissue levels of cyclic adenosine 3',5'-monophosphate.
我们研究了神经肽Y对安装在尤斯灌流小室中的新西兰白兔结肠黏膜条带的基础短路电流以及血管活性肠肽刺激引起的短路电流变化的影响。当将神经肽Y施加于基底外侧表面时，发现它会降低基础短路电流。神经肽Y通过一种对河豚毒素不敏感的机制，以浓度依赖的方式抑制血管活性肠肽刺激引起的短路电流增加。此外，神经肽Y还抑制了用福斯可林直接刺激腺苷酸环化酶所产生的短路电流增加。此外，神经肽Y可阻止血管活性肠肽刺激引起的组织环磷酸腺苷水平升高。这些结果表明，施加于基底外侧膜的神经肽Y通过一种对河豚毒素不敏感的机制抑制血管活性肠肽刺激引起的短路电流变化，该机制会降低组织中环磷酸腺苷3',5'-单磷酸的水平。
