Fingerle J, Au Y P, Clowes A W, Reidy M A
Department of Pathology, University of Washington, Seattle.
Arteriosclerosis. 1990 Nov-Dec;10(6):1082-7. doi: 10.1161/01.atv.10.6.1082.
Injury of an artery by passage of a balloon catheter causes both endothelial denudation and medial damage and produces a marked smooth muscle cell (SMC) proliferative response. In this study, the endothelium from rat carotid arteries was removed by use of a rotating loop of 5/0 monofilament suture (gentle denudation technique), which did not cause any detectable damage to the underlying medial cells but did cause platelet adherence. Expression of platelet-derived growth factor (PDGF) A-chain and PDGF receptor mRNA was comparable to that seen in ballooned carotids, but the medial SMC proliferative response to gentle denudation was markedly reduced when compared to that observed after balloon denudation (1.4% vs. 13.6%). Intimal lesions were only observed in those zones that remained denuded for more than 7 days. These results demonstrate that a denuding injury with no medial trauma is sufficient to induce intimal lesions and that the significantly higher proliferation seen in ballooned vessels might reflect a response of the medial cells to trauma that occurred during denudation.
球囊导管通过时对动脉造成的损伤会导致内皮剥脱和中层损伤，并产生明显的平滑肌细胞（SMC）增殖反应。在本研究中，使用5/0单丝缝线的旋转环（轻柔剥脱技术）去除大鼠颈动脉的内皮，该技术不会对下层中层细胞造成任何可检测到的损伤，但会导致血小板黏附。血小板衍生生长因子（PDGF）A链和PDGF受体mRNA的表达与球囊扩张颈动脉中的表达相当，但与球囊剥脱后观察到的情况相比，轻柔剥脱后中层SMC的增殖反应明显降低（1.4%对13.6%）。内膜病变仅在剥脱持续超过7天的区域观察到。这些结果表明，无中层创伤的剥脱性损伤足以诱导内膜病变，并且球囊扩张血管中明显更高的增殖可能反映了中层细胞对剥脱过程中发生的创伤的反应。
