NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country, Vitoria, Spain.
J Microencapsul. 2011;28(7):614-20. doi: 10.3109/02652048.2011.599439. Epub 2011 Jul 8.
Bovine serum albumin (BSA) loaded calcium alginate microparticles (MPs) produced in this study by a w/o emulsification and external gelation method exhibited spherical and fairly smooth and porous morphology with 1.052 ± 0.057 µm modal particle size. The high permeability of the calcium alginate hydrogel lead to a potent burst effect and too fast protein release. To overcome these problems, MPs were coated with polycations, such as chitosan, poly-L-lysine and DEAE-dextran. Our results demonstrated that coated MPs showed slower release and were able to significantly reduce the release of BSA in the first hour. Therefore, this method can be applied to prepare coated alginate MPs which could be an optimal system for the controlled release of biotherapeutic molecules. Nevertheless, further studies are needed to optimize delivery properties which could provide a sustained release of proteins.
本研究采用 w/o 乳化-外相固化法制备牛血清白蛋白（BSA）负载的海藻酸钠微球（MPs），所得 MPs 呈球形，表面光滑，具有多孔形貌，平均粒径为 1.052 ± 0.057 μm。海藻酸钠水凝胶具有高通透性，导致突释效应明显，蛋白释放过快。为解决这些问题，我们采用壳聚糖、聚赖氨酸和 DEAE-葡聚糖等聚阳离子对 MPs 进行包衣。结果表明，包衣 MPs 释放更缓慢，能够显著减少 BSA 在最初 1 小时的释放量。因此，该方法可用于制备包衣海藻酸钠 MPs，有望成为生物治疗分子控释的理想体系。然而，仍需进一步研究以优化其输送性能，从而实现蛋白的持续释放。
