Institute of Orthopaedics and Traumatology of PLA of China, Xijing Hospital, Shaanxi, China.
Cancer Sci. 2011 Oct;102(10):1774-81. doi: 10.1111/j.1349-7006.2011.02028.x. Epub 2011 Aug 3.
It has been proven that "side population (SP)" cells that exclude Hoechst 33342 dye are enriched with cancer stem cells in several tumors. In the present study we aimed to isolate and characterize SP cells from human primary osteosarcoma. Side population cells were detected in osteosarcoma samples. In vitro, SP cells regenerated both SP and non-SP and the clonogenicity of SP cells was higher than that of non-SP cells, just like stem cells. In vivo, SP cells exhibited heightened tumorigenicity and only the SP fraction had the capacity to self- renew both in vitro and in vivo. Furthermore, SP cells exhibited increased multidrug resistance and the RNA expression of ATP-binding cassette protein transporters was increased in the SP group. In addition, "stemness" genes Oct-4 and Nanog were also upregulated in the SP group. However, the expression of other putative stem cell markers (CD44, CD117 and CD133) had no significant difference between SP and non-SP for each individual marker. These findings suggest that SP cells derived from osteosarcoma are enriched with tumorigenic cells with stem-like properties and might be an ideal target for clinical therapy.
已有研究证明，在多种肿瘤中，排除 Hoechst 33342 染料的“侧群（SP）”细胞富含肿瘤干细胞。本研究旨在从人原发性骨肉瘤中分离和鉴定 SP 细胞。在骨肉瘤样本中检测到侧群细胞。体外，SP 细胞可再生 SP 细胞和非 SP 细胞，并且 SP 细胞的克隆形成能力高于非 SP 细胞，类似于干细胞。体内，SP 细胞表现出更高的致瘤性，只有 SP 部分具有在体外和体内自我更新的能力。此外，SP 细胞表现出增强的多药耐药性，并且 SP 组中 ATP 结合盒蛋白转运体的 RNA 表达增加。此外，“干性”基因 Oct-4 和 Nanog 在 SP 组中也上调。然而，对于每个单独的标记，SP 和非 SP 之间的其他假定干细胞标记物（CD44、CD117 和 CD133）的表达没有显著差异。这些发现表明，源自骨肉瘤的 SP 细胞富含具有干细胞样特性的致瘤细胞，可能是临床治疗的理想靶点。
