Brunel Institute for Bioengineering, Brunel University, Uxbridge UB83PH, UK.
J Chromatogr A. 2011 Sep 9;1218(36):6038-43. doi: 10.1016/j.chroma.2011.06.059. Epub 2011 Jun 22.
Calculation of the g-level is often used to compare CCC centrifuges, either against each other or to allow for comparison with other centrifugal techniques. This study shows the limitations of calculating the g-level in the traditional way. Traditional g-level calculations produce a constant value which does not accurately reflect the dynamics of the coil planet centrifuge. This work has led to a new equation which can be used to determine the improved non-dimensional values. The new equations describe the fluctuating radial and tangential g-level associated with CCC centrifuges and the mean radial g-level value. The latter has been found to be significantly different than that determined by the traditional equation. This new equation will give a better understanding of forces experienced by sample components and allows for more accurate comparison between centrifuges. Although the new equation is far better than the traditional equation for comparing different types of centrifuges, other factors such as the mixing regime may need to be considered to improve the comparison further.
g 值的计算常用于比较 CCC 离心机，无论是彼此之间的比较还是与其他离心技术的比较。本研究显示了传统 g 值计算方法的局限性。传统的 g 值计算会产生一个恒定的值，不能准确反映线圈行星离心机的动态。这项工作产生了一个新的方程，可以用来确定改进的无量纲值。新方程描述了与 CCC 离心机相关的波动的径向和切向 g 值以及平均径向 g 值。后者与传统方程确定的值有显著差异。这个新方程将更好地理解样品组件所经历的力，并允许更准确地比较离心机。虽然新方程在比较不同类型的离心机方面比传统方程要好得多，但可能需要考虑其他因素，如混合状态，以进一步提高比较的准确性。
