Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea.
Cancer Lett. 2011 Nov 1;310(1):53-60. doi: 10.1016/j.canlet.2011.06.013. Epub 2011 Jun 24.
A small animal imaging system for hepatocellular carcinoma (HCC)-specific reporter gene expression will enable monitoring of carcinogenesis or therapeutic intervention in vivo. Transgenic mouse was developed in which firefly luciferase (fLuc) expression was controlled by the AFP enhancer/promoter. The bioluminescent signals of the transgenic neonates were strong at their liver region and decreased after birth. Bioluminescent imaging (BLI) of a transgenic mouse treated with N-nitrosodiethylamine revealed distinct fLuc activity in the liver and an increased pattern with time. The transgenic mouse model can be used to monitor AFP producing HCC by a chemical carcinogen in a live animal by BLI.
一种用于肝细胞癌（HCC）特异性报告基因表达的小动物成像系统将能够在体内监测癌变或治疗干预。开发了一种转基因小鼠，其中萤火虫荧光素酶（fLuc）的表达受 AFP 增强子/启动子控制。转基因新生儿的生物发光信号在其肝脏区域很强，出生后减少。用 N-亚硝基二乙胺处理的转基因小鼠的生物发光成像（BLI）显示出肝脏中明显的 fLuc 活性，并随时间增加。该转基因小鼠模型可通过 BLI 在活体动物中监测化学致癌物诱导的 AFP 产生 HCC。
