Department of Radiology, Shengjing Hospital, China Medical University, Shenyang, China.
Nucl Med Biol. 2011 Nov;38(8):1205-12. doi: 10.1016/j.nucmedbio.2011.05.001. Epub 2011 Jul 7.
Alterations of dopamine in striatal presynaptic terminals play an important role in the hypoxic-ischemic (HI) brain injury. Quantification of DAT levels in the presynaptic site using (11)C-N-2-carbomethoxy-3-(4-fluorophenyl)-tropane ((11)C-CFT) with positron emission tomography (PET) was applied in studies for Parkinson's disease. The current study investigated the changes in striatal DAT following HI brain injury in newborn piglets using (11)C-CFT PET.
Newborn piglets were subjected to occlusion of bilateral common carotid arteries for 30 min and simultaneous peripheral hypoxia. Brain DAT imaging was performed using PET/CT with (11)C-CFT as the probe in each group (including the control group and HI insult groups). Brain tissues were collected for DAT immunohistochemical (IHC) analysis at each time point post the PET/CT procedure. Sham controls had some operation without HI procedure.
A few minutes after intravenous injection of (11)C-CFT, radioactive signals for DAT clearly appeared in the cortical area, striatum and cerebellum of newborn piglets of sham control group and HI insult groups. HI brain insult markedly increased striatal DAT at an early period (P<.05 vs. sham controls) when neuronal pathological changes were mild. Changes in striatal DAT were absent at later period post-HI insult when neuronal injury became more severe. (11)C-CFT PET imaging data and IHC DAT staining data were highly correlated (r=0.844, P<.05).
HI brain injury resulted in a transient increase in striatal DAT. (11)C-CFT PET/CT imaging data reflected the dynamic changes of DAT in the striatum in vivo.
纹状体内突触前末梢多巴胺的改变在缺氧缺血（HI）脑损伤中起着重要作用。使用正电子发射断层扫描（PET）中的（11）C-N-2-甲氧基-3-（4-氟苯基）-托烷（（11）C-CFT）对突触前部位的 DAT 水平进行定量，已应用于帕金森病的研究。本研究使用（11）C-CFT PET 研究新生仔猪 HI 脑损伤后纹状体 DAT 的变化。
新生仔猪双侧颈总动脉闭塞 30 分钟，同时外周缺氧。在每组（包括对照组和 HI 损伤组）中，使用 PET/CT 用（11）C-CFT 作为探针进行脑 DAT 成像。在 PET/CT 程序后的每个时间点收集脑组织进行 DAT 免疫组织化学（IHC）分析。假手术对照组仅进行一些无 HI 程序的操作。
静脉注射（11）C-CFT 几分钟后，放射性信号清晰地出现在皮质区、纹状体和小脑。HI 脑损伤在早期（P<.05 与假手术对照组相比）明显增加了纹状体 DAT，此时神经元病理变化较轻。HI 损伤后晚期，当神经元损伤变得更严重时，纹状体 DAT 的变化消失。（11）C-CFT PET 成像数据和 IHC DAT 染色数据高度相关（r=0.844，P<.05）。
HI 脑损伤导致纹状体 DAT 短暂增加。（11）C-CFT PET/CT 成像数据反映了纹状体中 DAT 的动态变化。
