US Department of Agriculture, Agriculture Research Service, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL 32608, USA.
J Invertebr Pathol. 2011 Sep;108(1):40-5. doi: 10.1016/j.jip.2011.06.008. Epub 2011 Jun 29.
Mosquito iridescent viruses (MIV) are members of the genus Chloriridovirus that currently contains only the type IIV-3 from Aedestaeniorhynchus. The complete genome of invertebrate iridescent virus -3 (IIV-3) has been sequenced and the availability of a tissue culture system would facilitate functional genomic studies. This investigation, using quantitative PCR and electron microscopy, has determined that the mosquito cell lines Aedes aegypti (Aag2), Aedes albopictus (C6/36) and Anopheles gambiae (4a3A) as well as the lepidopteran cell line from Spodoptera frugiperda (SF9) are permissive to IIV-3 infection. However, IIV-3 infection remained longer in Aag2 and C6/36 cells. Virus produced in C6/36 cell line was infectious to larvae of A. taeniorhynchus by injection and per os. Ultrastructural examination of 4a3A and SF9 cells infected with IIV-3 revealed an unusual feature, where virions were localized to mitochondria. It is speculated that containment with mitochondria may play a role in the lack of persistence in these cell lines.
蚊虫虹彩病毒（MIV）是质型多角体病毒科的一个属，目前仅包含来源于致倦库蚊的 I 型蚊虫虹彩病毒-3（IIV-3）。昆虫虹彩病毒-3（IIV-3）的全基因组序列已被测序，组织培养系统的建立将有利于开展功能基因组学研究。本研究采用定量 PCR 和电子显微镜技术，确定了埃及伊蚊（Aag2）、白纹伊蚊（C6/36）和冈比亚按蚊（4a3A）等蚊细胞系以及鳞翅目昆虫细胞系 Spodoptera frugiperda（SF9）均允许 IIV-3 感染。然而，IIV-3 在 Aag2 和 C6/36 细胞中的感染持续时间更长。用 C6/36 细胞系生产的病毒可通过注射和口服感染致倦库蚊幼虫。用 IIV-3 感染 4a3A 和 SF9 细胞的超微结构检查显示出一个不寻常的特征，即病毒粒子定位于线粒体。推测与线粒体的包含可能在这些细胞系中缺乏持续性方面发挥作用。
