Department of Scienze Agro-Ambientali e Territoriali, Università degli Studi di Bari Aldo Moro, Via G. Amendola 165/A, Bari, Italy.
Anim Reprod Sci. 2011 Jul;126(3-4):187-94. doi: 10.1016/j.anireprosci.2011.05.011. Epub 2011 May 27.
Two experiments were carried out on Ionica dairy goats in order to test the efficiency of: (1) short term-5-day combined progestogen-PGF2α-GnRH treatments on induction/synchronization of oestrus and fertility after natural mating in lactating goats and during the transition period (Experiment 1); (2) short term-9-day FGA-PGF2α-eCG treatments on synchronizing oestrus and ovulation (Experiment 2.1) and artificial insemination (AI) fixed time system in synchronized does (Experiment 2.2), during the breeding season. In Experiment 1, four treatment groups (N=24) were considered: (1) FPe-11d - control, FGA intravaginal sponges (11 days)+PGF2α (9th d)+eCG (11th d); (2) FPe-5d, FGA (5 days)+PGF2α (5th d)+eCG (5th d); (3) PFe-5d, PGF2α (D0)+FGA (5 days)+eCG (5th d); (4) GPe-5d, GnRH (D0)+PGF2α (5th d)+eCG (5th d). Goats were checked for oestrus and naturally mated. The occurrence of oestrus was 75.0, 78.3, 86.4, and 58.3% for groups 1-4, respectively, with significant differences (P<0.05) between groups 3 and 4. Interval to oestrus was earlier (P<0.05) in GPE-5d than in FPe-11d control group. There were no differences between the groups (P>0.05) in fertility or in prolificacy. In Experiment 2.1, 22 goats were subdivided into two treatment groups (N=11): (T1) FPe-11d (control), FGA (11 days)+PGF2α (9th d)+eCG (11th d); (T2) FPe-9d, FGA (9 days)+PGF2α (7th d)+eCG (9th d). Oestrus and ovulation times were monitored every 4h; ovulation rate was also determined. The induction of oestrus ranged from 91 to 100% and all goats ovulated. Intervals to oestrus, from the onset of oestrus to ovulation, from sponge removal to ovulation, and ovulation rates were 28.2±4.9 and 26.0±4.0h, 25.3±9.2 and 28.9±7.4h, 53.5±7.6 and 54.9±7.1h, 3.7±1.6 and 2.4±1.4 corpora lutea (P<0.05) for T1 and T2, respectively. In T2 a great abnormal ovulatory response was observed. In Experiment 2.2, 48 goats were synchronized with FPe-9d treatment and subjected to AI, performed 50h after s.r. with frozen semen, and subdivided into 2 AI system groups (N=24): T3, exocervical AI (100×10(6)Spz/doe); T4, intrauterine AI (20×10(6)Spz/doe). Fertility rate was higher (P<0.05) in T4. It seems that short term-5-day combined progestogen-PGF2α-GnRH-eCG treatments need to be investigated for AI fixed time.
两项实验在 Ionica 奶山羊上进行，旨在检验以下方法的效率：（1）在哺乳期和过渡期间，短期 5 天孕激素-PGF2α-GnRH 联合处理对自然交配后发情和生育力的诱导/同步作用（实验 1）；（2）短期 9 天 FGA-PGF2α-eCG 处理对发情和排卵的同步作用（实验 2.1）以及同步发情母羊的人工授精（AI）固定时间系统（实验 2.2），在繁殖季节。在实验 1 中，考虑了四个处理组（N=24）：（1）FPe-11d-对照，FGA 阴道海绵（11 天）+PGF2α（第 9 天）+eCG（第 11 天）；（2）FPe-5d，FGA（5 天）+PGF2α（第 5 天）+eCG（第 5 天）；（3）PFe-5d，PGF2α（D0）+FGA（5 天）+eCG（第 5 天）；（4）GPe-5d，GnRH（D0）+PGF2α（第 5 天）+eCG（第 5 天）。检查山羊发情并自然交配。第 1-4 组的发情率分别为 75.0%、78.3%、86.4%和 58.3%，第 3 组和第 4 组之间存在显著差异（P<0.05）。与 FPe-11d 对照组相比，GPE-5d 的发情间隔更早（P<0.05）。各组间的生育力或繁殖力均无差异（P>0.05）。在实验 2.1 中，将 22 只山羊分为两组（N=11）：（T1）FPe-11d（对照），FGA（11 天）+PGF2α（第 9 天）+eCG（第 11 天）；（T2）FPe-9d，FGA（9 天）+PGF2α（第 7 天）+eCG（第 9 天）。每 4 小时监测发情和排卵时间；也确定了排卵率。发情诱导率为 91%至 100%，所有山羊均排卵。从发情开始到排卵、从海绵去除到排卵的发情间隔、排卵率分别为 28.2±4.9 和 26.0±4.0h、25.3±9.2 和 28.9±7.4h、53.5±7.6 和 54.9±7.1h、3.7±1.6 和 2.4±1.4 黄体（P<0.05），分别为 T1 和 T2。在 T2 中观察到了很大的异常排卵反应。在实验 2.2 中，用 FPe-9d 处理对 48 只山羊进行同步处理，并进行了人工授精，在冷冻精液阴道内输精后 50h 进行，分为 2 个 AI 系统组（N=24）：T3，宫颈外 AI（100×10(6)Spz/doe）；T4，子宫内 AI（20×10(6)Spz/doe）。T4 的受胎率更高（P<0.05）。似乎需要研究短期 5 天孕激素-PGF2α-GnRH-eCG 联合处理对 AI 固定时间的作用。
