Department of Cell Physiology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Biosens Bioelectron. 2011 Sep 15;27(1):34-9. doi: 10.1016/j.bios.2011.05.041. Epub 2011 Jun 1.
We measured biomagnetic fields in musculatures with spontaneous electric activity using a pulse-driven magnetoimpedance (PMI) sensor with the sensitivity improved toward a pico-Tesla (pT) level. Due to the sufficiently short operation interval of 1 μs, this magnetic sensor enabled quasi-real time recordings of the magnetic field for biological electric activity. Isolated small musculatures from the guinea-pig stomach, taenia caeci, portal vein and urinary bladder were incubated in an organ bath at a body temperature. The improved PMI sensor mounted approximately 1mm below the preparations detected oscillatory magnetic fields reflecting spontaneous electric activities of musculature preparations. In the taenia caeci, application of tetraethyl ammonium (TEA), a K(+) channel blocker, significantly enhanced the magnetic activity estimated by histogram analysis. Also, in some musculature preparations, simultaneous measurements with electric activity revealed that the observed magnetic activities were attributed to biological electric activity. PMI technology is promising for applications in biology and medicine.
我们使用灵敏度提高到皮特斯拉（pT）级别的脉冲驱动磁阻抗（PMI）传感器测量具有自发电活动的肌肉中的生物磁场。由于操作间隔足够短，只有 1μs，因此该磁传感器能够对生物电活动的磁场进行准实时记录。从小鼠胃、盲肠纵肌、门静脉和膀胱中分离出的小型肌肉组织在体温下的器官浴中孵育。安装在大约 1mm 下方的改进型 PMI 传感器检测到反映肌肉组织自发电活动的振荡磁场。在盲肠纵肌中，钾离子通道阻断剂四乙铵（TEA）的应用显著增强了通过直方图分析估计的磁活性。此外，在一些肌肉组织标本中，与电活动的同时测量表明，观察到的磁活性归因于生物电活动。PMI 技术在生物学和医学中的应用具有广阔的前景。
