荧光相关光谱法研究抗污 PEG 化表面的渗透性。
Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy.
机构信息
Department of Chemistry, Rice University, Houston, TX 77251, United States.
出版信息
Colloids Surf B Biointerfaces. 2011 Nov 1;88(1):31-8. doi: 10.1016/j.colsurfb.2011.05.044. Epub 2011 May 27.
Abstract
The present work reports on in situ observations of the interaction of organic dye probe molecules and dye-labeled protein with different poly(ethylene glycol) (PEG) architectures (linear, dendron, and bottle brush). Fluorescence correlation spectroscopy (FCS) and single molecule event analysis were used to examine the nature and extent of probe-PEG interactions. The data support a sieve-like model in which size-exclusion principles determine the extent of probe-PEG interactions. Small probes are trapped by more dense PEG architectures and large probes interact more with less dense PEG surfaces. These results, and the tunable pore structure of the PEG dendrons employed in this work, suggest the viability of electrochemically-active materials for tunable surfaces.
摘要
本工作报道了有机染料探针分子和染料标记蛋白与不同聚乙二醇（PEG）结构（线性、树枝状和刷状）相互作用的原位观察结果。荧光相关光谱（FCS）和单分子事件分析用于研究探针-PEG 相互作用的性质和程度。数据支持一种筛状模型，其中尺寸排阻原理决定了探针-PEG 相互作用的程度。小探针被更密集的 PEG 结构捕获，而大探针与密度较低的 PEG 表面相互作用更多。这些结果以及本工作中使用的 PEG 树枝状分子的可调孔径结构表明，电化学活性材料在可调表面方面具有可行性。
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