Department of Physiology, Tianjin University of Traditional Medicine, Tianjin 300193, China.
Acta Biochim Biophys Sin (Shanghai). 2011 Aug;43(8):601-6. doi: 10.1093/abbs/gmr057. Epub 2011 Jul 8.
The purpose of the present study was to characterize the expressions of phosphorylated Ca(2+)/calmodulin-dependent protein kinase II (p-CaMK-II), total CaMK-II, and L-type Ca(2+) channel in offspring hippocampus that was induced by prenatal restraint stress. Pregnant rats were divided into two groups: the control group and the prenatal stress (PNS) group. Pregnant rats in the PNS group were exposed to restraint stress on day 14-20 of pregnancy three times daily for 45 min. Adult offspring rats were used in this study. The results demonstrated that prenatal restraint stress induced a significant increase in the expression of p-CaMK-II, total CaMK-II, and L-Ca(2+) channel by western blot analysis in offspring hippocampus. The immunohistochemistry results revealed that PNS increased the expressions of CaMK-II and L-Ca(2+) channel in the hippocampal CA3 of offspring rats. These data suggest that PNS can have long-term neuronal effects within hippocampal structure involved in the feedback mechanisms of the hypothalamo-pituitary-adrenal axis.
本研究旨在描述产前束缚应激诱导的子代海马中磷酸化钙/钙调蛋白依赖性蛋白激酶 II（p-CaMK-II）、总 CaMK-II 和 L 型钙通道的表达情况。将怀孕大鼠分为两组：对照组和产前应激（PNS）组。PNS 组的怀孕大鼠在妊娠第 14-20 天每天接受 3 次 45 分钟的束缚应激。本研究使用成年子代大鼠。结果表明，通过 Western blot 分析，产前束缚应激诱导子代海马中 p-CaMK-II、总 CaMK-II 和 L-Ca(2+)通道的表达显著增加。免疫组织化学结果显示，PNS 增加了子代大鼠海马 CA3 区 CaMK-II 和 L-Ca(2+)通道的表达。这些数据表明，PNS 可以对参与下丘脑-垂体-肾上腺轴反馈机制的海马结构中的神经元产生长期影响。
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