[碘缺乏和甲状腺功能减退对幼崽海马中CaMK II蛋白表达的影响]

[Effect of iodine deficiency and hypothyroidism on the protein expressions of CaMK II in the hippocampus of pups].

作者信息

Wei Wei, Dong Jing, Liu Wanyang, Wang Yi, Chen Jie

机构信息

Department of Occupational and Environmental Health, School of Public Health, China Medical University, Shenyang 110001, China.

出版信息

Wei Sheng Yan Jiu. 2010 Mar;39(2):180-3.

PMID:20459031

Abstract

OBJECTIVE

To observe the effect of iodine deficiency and hypothyroidism on the protein expressions of CaMK II in the hippocampus of pups.

METHODS

Female Wistar rats (n=28) after pregnancy were randomly divided into control group, hypothyroid group and iodine deficient group. According to the dose of PTU in fed water, hypothyroid group was divided into 5 mg/L group and 15 mg/L group (7 in each). 5 pups from each group were sacrificed and perfused intracardially in PN7, PN14 and PN21. Brains were removed, fixed and sectioned coronally. All sections were observed and analyzed the protein exression of CaMK II by immunohistochemistry in the hippocampus CA1, CA3 and DG regions.

RESULTS

Control group in CA1, CA3 and DG regions of the hippocampus had strong positive staining and the cytoplasm of neurons were filled with CaMK II. The distribution of the immune reaction product in hippocampus of 5 mg/L, 15 mg/L and iodine groups were in line with the control group, but the staining intensity as compared with the control group gradually decreased. In PN21 and PN14, integrated optical density average of CaMK II in CA1, CA3 and DG regions of the hippocampus in iodine deficient group [PN21: (26.05 +/- 4.98), (30.79 +/- 3.22), (26.40 +/- 2.63); PN14:(25.48 +/- 4.87), (44.17 +/- 5.91), (26.41 +/- 3.01)] and 15 mg/L groups [PN21: (17.02 +/- 2.68), (24.57 +/- 6.62), (20.18 +/- 4.05); PN14:(20.66 +/- 3.51), (34.94 +/- 5.09), (27.32 +/- 4.97)] were significantly lower than those of controls [PN21: (57.75 +/- 13.22), (65.03 +/- 6.20), (49.39 +/- 8.41), P < 0.05; PN14: (54.08 +/- 12.00), (71.04 +/- 5.07), (78.52 +/- 12.42), P < 0.05]. In PN7, integrated optical density average of CaMK II in CA1 region of the hippocampus in iodine deficient group (25.74 +/- 3.33) and 15 mg/L groups (26.89 +/- 5.25) were significantly lower than those of controls(40.53 +/- 3.65), P < 0.05.

CONCLUSION

Iodine deficiency and hypothyroidism may decrease the protein expression of CaMK II.

摘要

目的

观察碘缺乏和甲状腺功能减退对幼鼠海马中CaMK II蛋白表达的影响。

方法

将28只妊娠后的雌性Wistar大鼠随机分为对照组、甲状腺功能减退组和碘缺乏组。根据饮水中PTU的剂量，甲状腺功能减退组分为5 mg/L组和15 mg/L组（每组7只）。在出生后第7天（PN7）、第14天（PN14）和第21天（PN21），每组处死5只幼鼠并进行心脏灌注。取出大脑，固定并冠状切片。所有切片经免疫组织化学法观察并分析海马CA1、CA3和齿状回（DG）区域中CaMK II的蛋白表达。

结果

对照组海马CA1、CA3和DG区域呈强阳性染色，神经元胞质中充满CaMK II。5 mg/L组、15 mg/L组和碘缺乏组海马中免疫反应产物的分布与对照组一致，但与对照组相比染色强度逐渐降低。在PN21和PN14时，碘缺乏组[PN21：（26.05±4.98），（30.79±3.22），（26.40±2.63）；PN14：（25.48±4.87），（44.17±5.91），（26.41±3.01）]和15 mg/L组[PN21：（17.02±2.68），（24.57±6.62），（20.18±4.05）；PN14：（20.66±3.51），（34.94±5.09），（27.32±4.97）]海马CA1、CA3和DG区域CaMK II的积分光密度平均值显著低于对照组[PN21：（57.75±13.22），（65.03±6.20），（49.39±8.41），P<0.05；PN14：（54.08±12.00），（71.04±5.07），（78.52±12.42），P<0.05]。在PN7时，碘缺乏组（25.74±3.33）和15 mg/L组（26.89±5.25）海马CA1区域CaMK II的积分光密度平均值显著低于对照组（40.53±3.65），P<0.05。