Fu Y
Zhonghua Yi Xue Za Zhi. 1990 Aug;70(8):442-5, 32.
The presence of an endogenous ouabain-like substance in bovine hypothalamus is demonstrated on the bas is of the ability of acid acetone extracts of hypothalamus to inhibit (NA+ + K+)-ATPase activity, 86Rb uptake, 22Na efflux and (3H)ouabain binding. Partial purification of the substance was achieved by acetic acid fractionation followed by sephadex-G25 chromatography and subsequent desalting by mixed-bed resin. The results are discussed with relation to the important role of the sodium pump inhibitor in the regulation of (N+ + K+)-ATPase activity and sodium transport and hence in the pathophysiologic mechanisms of essential hypertension.
基于下丘脑酸性丙酮提取物抑制（Na⁺ + K⁺）-ATP酶活性、⁸⁶Rb摄取、²²Na外流以及（³H）哇巴因结合的能力，证明了牛下丘脑存在内源性哇巴因样物质。通过乙酸分级分离，随后进行葡聚糖凝胶G-25柱层析以及用混合床树脂脱盐，实现了该物质的部分纯化。结合钠泵抑制剂在调节（Na⁺ + K⁺）-ATP酶活性和钠转运中的重要作用，进而在原发性高血压病理生理机制中的作用，对结果进行了讨论。
