Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, 32 Creelman St., Mississippi State, MS 39762, USA.
Appl Environ Microbiol. 2011 Sep;77(17):6189-98. doi: 10.1128/AEM.00377-11. Epub 2011 Jul 8.
A striking feature of Burkholderia contaminans strain MS14 is the production of a glycolipopeptide named occidiofungin. Occidiofungin has a broad range of antifungal activities against plant and animal pathogens. In this study, a complete covalent structure characterization and identification of the whole genomic DNA region for the occidiofungin gene (ocf) cluster are described. Discovery of the presence of 2,4-diaminobutyric acid and 3-chloro-β-hydroxytyrosine and elucidation of the structure of a novel C(18) fatty amino acid residue have been achieved. In addition, seven additional putative open reading frames (the genes from ocfI to ocfN [ocfI-N] and ORF16) were identified. Transcription of all the putative genes ocfI-N identified in the region except ORF16 was regulated by both ambR1 and ambR2. Elucidation of the structure and the ocf gene cluster provides insight into the biosynthesis of occidiofungin and promotes future aims at understanding the biosynthetic machinery. This work provides new avenues for optimizing the production and synthesis of structural analogs of occidiofungin.
被污染伯克霍尔德氏菌菌株 MS14 的一个显著特征是产生一种名为球腔菌素的糖脂肽。球腔菌素对植物和动物病原体具有广泛的抗真菌活性。在本研究中，描述了球腔菌素基因（ocf）簇的完整共价结构特征和整个基因组 DNA 区域的鉴定。发现了 2,4-二氨基丁酸和 3-氯-β-羟基酪氨酸的存在，并阐明了一种新型 C(18)脂肪酸氨基酸残基的结构。此外，还鉴定了七个额外的推定开放阅读框（ocfI 到 ocfN [ocfI-N] 和 ORF16 基因）。该区域中除 ORF16 外的所有推定基因 ocfI-N 的转录均受 ambR1 和 ambR2 的调节。球腔菌素结构和 ocf 基因簇的阐明为球腔菌素的生物合成提供了深入的了解，并促进了未来对生物合成机制的理解。这项工作为优化球腔菌素的生产和合成提供了新的途径。
